Deciphering the ABA and GA biosynthesis approach of Bacillus pumilus, mechanistic approach, explaining the role of metabolic region as an aid in improving the stress tolerance.

Autor: Shaffique S; School of Applied Biosciences, Kyungpook National University, Daegu, South Korea., Shah AA; Department of Botany, Division of Science and Technology, University of Education, Lahore, Pakistan., Odongkara P; School of Applied Biosciences, Kyungpook National University, Daegu, South Korea., Elansary HO; Prince Sultan Bin Abdulaziz International Prize for Water Chair, Prince Sultan Institute for Environmental, Water and Desert Research, King Saud University, Riyadh 11451, Saudi Arabia., Khan AL; Department of Biology and Biochemistry, University of Houston, Houston, TX, USA.; Department of Engineering Technology, University of Houston, Sugar Land, TX, USA., Adhikari A; School of Applied Biosciences, Kyungpook National University, Daegu, South Korea., Kang SM; School of Applied Biosciences, Kyungpook National University, Daegu, South Korea., Lee IJ; School of Applied Biosciences, Kyungpook National University, Daegu, South Korea. ijlee@knu.ac.kr.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Nov 22; Vol. 14 (1), pp. 28923. Date of Electronic Publication: 2024 Nov 22.
DOI: 10.1038/s41598-024-78227-3
Abstrakt: Bacillus pumilus plays an essential role in agricultural applications as a beneficial microbe and for sustainable agriculture production. However, the underlying mechanisms of B. pumilus strains remain unclear as to how they are beneficial for plants as stress tolerant and growth promoters. Bacillus pumilus was isolated from the rhizosphere soil of Artemisia vulgaris. NGS (next-generation sequencing) was performed for the strain to gain new insights into the molecular mechanisms underlying plant-microbial interactions. NGS revealed 3,910 genes, 3294 genes with protein-coding, and 11 functional genomic regions related to diverse agronomic traits including stress tolerance. We identified the two possible phytohormone biosynthesis approaches from metabolic regions1(terpense→diterpense→betacarotene→xanthoxin→ABA)2(terpense→diterpense→geranyl diphosphate →C20 →GA). Several gene clusters related to the biosynthesis of phytohormones, stress tolerance, and agricultural diversification were predicted. The genome provides insights into the possible mechanisms of this bacterium for stress tolerance and its future applications. The genomic organization of B. pumilus revealed several hallmarks of its plant growth promotion and pathogen suppression activities. Our results provide detailed genomic information for the strain and reveal its potential stress tolerance mechanisms, laying the foundation for developing effective stress tolerance strategies against abiotic stress.
Competing Interests: Declarations. Competing interests: The authors declare no competing interests.
(© 2024. The Author(s).)
Databáze: MEDLINE
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