The impact of skinfold thickness and exercise intensity on the reliability of NIRS in the vastus lateralis.

Autor: Stuer L; Department of Movement and Sport Sciences, Ghent University, Watersportlaan 2, 9000, Ghent, Belgium.; Univ. Lille, Univ. Artois, Univ. Littoral Côte d'Opale, ULR, URePSSS-Unité de Recherche Pluridisciplinaire Sport Santé Société, 7369, 59000, Lille, France., Teso M; Department of Neurosciences, Biomedicine and Movement Sciences, University of Verona, Verona, Italy., Colosio AL; Department of Movement and Sport Sciences, Ghent University, Watersportlaan 2, 9000, Ghent, Belgium., Loi M; Department of Neurosciences, Biomedicine and Movement Sciences, University of Verona, Verona, Italy., Mucci P; Univ. Lille, Univ. Artois, Univ. Littoral Côte d'Opale, ULR, URePSSS-Unité de Recherche Pluridisciplinaire Sport Santé Société, 7369, 59000, Lille, France., Pogliaghi S; Department of Neurosciences, Biomedicine and Movement Sciences, University of Verona, Verona, Italy., Boone J; Department of Movement and Sport Sciences, Ghent University, Watersportlaan 2, 9000, Ghent, Belgium. jan.boone@UGent.be., Caen K; Department of Movement and Sport Sciences, Ghent University, Watersportlaan 2, 9000, Ghent, Belgium.
Jazyk: angličtina
Zdroj: European journal of applied physiology [Eur J Appl Physiol] 2024 Nov 21. Date of Electronic Publication: 2024 Nov 21.
DOI: 10.1007/s00421-024-05654-5
Abstrakt: Purpose: The aims of this study were (1) to assess the test-retest reliability of the primary near infrared spectroscopy (NIRS) variables (i.e., StO 2 , T[Hb], [HbO 2 ] and [HHb]) during cycling and (2) to investigate potential influences of exercise intensity and adipose tissue thickness (ATT) on this reliability.
Methods: 21 men and 20 women completed twelve constant work rate tests (6 min) at six different exercise intensities with each intensity performed twice. NIRS variables were measured at the vastus lateralis. The coefficient of variance (CV%), the intraclass correlation coefficient (ICC), mean bias and limits of agreement (LoA) were determined for reliability purposes.
Results: The reliability of baseline values were acceptable to very good (CV% range: 5.83 - 21.96%). The reliability of end-values (CV% range: 0.02 - 25.02%, ICC range: 0.0 - 0.935) and amplitudes (CV% range: 0.46 - 5099%, ICC range: 0.0 - 0.887) were more variable. In general, the mean biases of end-values and amplitudes showed wide limits of agreement. A homogeneous influence of exercise intensity on reliability could not be established but reliability measures appeared to be lower in people with a lower skinfold thickness. Moreover, the NIRS signals decreased with increasing ATT but stabilized upon reaching a cut-off of 8 mm ATT. In addition, ATT did have a significant influence on [HHb] amplitude. In participants with ATT < 8 mm, higher amplitudes were observed with increasing intensity whereas in participants with ATT > 8 mm, there were no differences between the intensities.
Conclusion: The study reveals variable results with regards to reliability and there was no consistent influence of exercise intensity on reliability. Participants with a lower skinfold thickness showed stronger reliability. Moreover, NIRS signals decrease when ATT exceeds 8 mm. Careful consideration is necessary when interpreting NIRS signals in such cases.
Competing Interests: Declarations. Conflict of interest: Results of the present study are presented clearly, honestly and without fabrication, falsifcation or inappropriate data manipulation. No conficts of interest, fnancial or otherwise, are declared by the authors. Ethical approval: This study was approved by the Ethical Committee of the Ghent University Hospital (Ghent, Belgium) and the University of Verona Ethics Committee for Research on Human Subjects (Verona, Italy) in compliance with de Declaration of Helsinki (1964). Consent to participate: Informed consent for participation in this study was received from all subjects. Consent for publication: Consent for publication of the current study results was received from all subjects.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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