Structural characterization of a polysaccharide from Scutellaria baicalensis Georgi and its immune-enhancing properties on RAW264.7 cells.

Autor:	Xiong S; The MOE Key Laboratory for Standardization of Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Mate ria Medica, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, PR China., Li N; Longhua Hospital, Shanghai University of Traditional Chinese Medicine, 725 Wan-Ping South Road, Shanghai 200032, China; Spine Institute, Shanghai University of Traditional Chinese Medicine, 725 Wan-Ping South Road, Shanghai 200032, China., Shi S; The MOE Key Laboratory for Standardization of Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Mate ria Medica, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, PR China., Zhao Y; The MOE Key Laboratory for Standardization of Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Mate ria Medica, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, PR China., Chen J; The MOE Key Laboratory for Standardization of Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Mate ria Medica, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, PR China., Ruan M; Longhua Hospital, Shanghai University of Traditional Chinese Medicine, 725 Wan-Ping South Road, Shanghai 200032, China; Spine Institute, Shanghai University of Traditional Chinese Medicine, 725 Wan-Ping South Road, Shanghai 200032, China., Xu Y; The MOE Key Laboratory for Standardization of Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Mate ria Medica, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, PR China., Liu R; The MOE Key Laboratory for Standardization of Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Mate ria Medica, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, PR China., Wang S; The MOE Key Laboratory for Standardization of Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Mate ria Medica, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, PR China. Electronic address: shunchunwang@126.com., Wang H; The MOE Key Laboratory for Standardization of Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Mate ria Medica, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, PR China. Electronic address: huijun.wang@outlook.com.
Jazyk:	angličtina
Zdroj:	International journal of biological macromolecules [Int J Biol Macromol] 2024 Dec; Vol. 283 (Pt 3), pp. 137890. Date of Electronic Publication: 2024 Nov 19.
DOI:	10.1016/j.ijbiomac.2024.137890
Abstrakt:	A novel polysaccharide SPS01-2 (87.5 kDa) was isolated from the roots of Scutellaria baicalensis Georgi. Monosaccharide composition revealed that SPS01-2 consists of rhamnose, arabinose, galactose, galacturonic acid, and glucuronic acid in ratio of 4.4: 67.1: 22.2: 6.3: 1.2. Further investigations using methylation, NMR, and mass spectrometry indicated that SPS01-2 is classified as a type II arabinogalactan (AG-II) with a minor presence of type I rhamnogalacturonan (RG-I). The core structure alternates between 1,2/1,2,4-α-L-Rhap and 1,4-α-D-GalpA, with branches including 1,3,6-β-D-Galp, 1,3-β-D-Galp, T-β-D-Galp, and T-α-L-Rhap. The RG-I regions are linked to 1,6-β-D-Galp, and 1,3,6-β-D-Galp units. Numerous arabinan branches, featuring multiple branching points, are attached to the O-3 position of galactose. Additionally, T-β-D-Galp, 1,6-β-D-Galp, and T-β-D-4-OMe-GlcpA are also linked to galactose in the backbone. Furthermore, SPS01-2 demonstrated potential immune-enhancing properties by dose-dependently increasing proliferation, phagocytosis, and the production of nitric oxide and cytokines (TNF-α, IL-6, and IL-1β) in RAW264.7 cells. It also enhanced the expression of CD80, CD86, and MHC-II at concentrations ranging from 5 to 200 μg/mL. Moreover, the immunostimulatory activity of SPS01-2 was significantly reduced when branch linkages were removed through partial acid hydrolysis. Our findings indicate that SPS01-2 could serve as a natural immunostimulant in the food and pharmaceutical sectors. Competing Interests: Declaration of competing interest The authors declared no potential conflicts of interest with respect to the research, author-ship, and/or publication of this article. (Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze:	MEDLINE
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