Chemically induced proximity reveals a Piezo-dependent meiotic checkpoint at the oocyte nuclear envelope.

Autor:	Liu C; California Institute for Quantitative Biosciences (QB3) and Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, CA, USA.; Division of Biological Systems and Engineering, Lawrence Berkeley National Laboratory, Berkeley, CA, USA., Dernburg AF; California Institute for Quantitative Biosciences (QB3) and Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, CA, USA.; Division of Biological Systems and Engineering, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
Jazyk:	angličtina
Zdroj:	Science (New York, N.Y.) [Science] 2024 Nov 22; Vol. 386 (6724), pp. eadm7969. Date of Electronic Publication: 2024 Nov 22.
DOI:	10.1126/science.adm7969
Abstrakt:	Sexual reproduction relies on robust quality control during meiosis. Assembly of the synaptonemal complex between homologous chromosomes (synapsis) regulates meiotic recombination and is crucial for accurate chromosome segregation in most eukaryotes. Synapsis defects can trigger cell cycle delays and, in some cases, apoptosis. We developed and deployed a chemically induced proximity system to identify key elements of this quality control pathway in Caenorhabditis elegans . Persistence of the polo-like kinase PLK-2 at pairing centers-specialized chromosome regions that interact with the nuclear envelope-induced apoptosis of oocytes in response to phosphorylation and destabilization of the nuclear lamina. Unexpectedly, the Piezo1/PEZO-1 channel localized to the nuclear envelope and was required to transduce this signal to promote apoptosis in maturing oocytes.
Databáze:	MEDLINE
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