| Autor: |
Raddaoui A; Laboratory Ward, National Bone Marrow Transplant Center, Tunis, Tunisia.; Faculty of Medicine of Tunis, Laboratory LR18ES39, University of Tunis El Manar, Tunis, Tunisia., Chebbi Y; Laboratory Ward, National Bone Marrow Transplant Center, Tunis, Tunisia.; Faculty of Medicine of Tunis, Laboratory LR18ES39, University of Tunis El Manar, Tunis, Tunisia., Frigui S; Laboratory Ward, National Bone Marrow Transplant Center, Tunis, Tunisia.; Faculty of Medicine of Tunis, Laboratory LR18ES39, University of Tunis El Manar, Tunis, Tunisia., Ammeri RW; Laboratory Ward, National Bone Marrow Transplant Center, Tunis, Tunisia.; Faculty of Medicine of Tunis, Laboratory LR18ES39, University of Tunis El Manar, Tunis, Tunisia., Ben Abdejlil N; Department of Hematology and Transplantation, National Bone Marrow Transplant Center, Tunis, Tunisia., Abbassi MS; Faculty of Medicine of Tunis, Laboratory of Antibiotic Resistance LR99ES09, University of Tunis El Manar, Tunis, Tunisia.; Institute of Veterinary Research of Tunisia, University of Tunis El Manar, Tunis, Tunisia., Achour W; Laboratory Ward, National Bone Marrow Transplant Center, Tunis, Tunisia.; Faculty of Medicine of Tunis, Laboratory LR18ES39, University of Tunis El Manar, Tunis, Tunisia. |
| Abstrakt: |
This study aimed to characterize the first vancomycin-resistant Enterococcus faecalis (VREfs) isolate from patient with neutropenic in Tunisia by whole-genome sequencing (WGS). This strain was detected from routine rectal swab from an 8-year-old child with bone marrow aplasia, residing in a rural area, on September 20, 2021. The strain was isolated after 12 days of hospitalization at the National Bone Marrow Transplant Center. Minimum Inhibitory Concentrations of vancomycin and teicoplanin were >256 and 16 mg/L, respectively. WGS revealed that the strain belonged to the ST249 clone, exclusively reported in avian (poultry and ducks) vancomycin-susceptible E. faecalis isolates in six studies from four countries, primarily Denmark. The vanA gene was carried by the Tn 1546 transposon mobilized by a pTW9-like plasmid. The ardA gene, a CRISPR-Cas system neutralization factor, was detected in this strain. In summary, this is the first report of avian-associated E. faecalis ST249 in clinical samples. Initially vancomycin susceptible, the strain acquired a pTW9-like plasmid carrying the classical vanA -Tn 1546 transposon. This acquisition was facilitated by the sex pheromone-response mechanisms and the ard A gene and CRISPR-Cas system neutralization. |
