Gastrodin Attenuates Neuroinflammation and Injury in Young Rats with LiCl/Pilocarpine-Induced Status Epilepticus.

Autor: Dai J; Department of Neurology, Nantong First People's Hospital, Nantong, 226014, Jiangsu, China., Shen HL; Department of Neurology, Nantong First People's Hospital, Nantong, 226014, Jiangsu, China., Li J; Department of Neurology, Nantong First People's Hospital, Nantong, 226014, Jiangsu, China., Zhou Y; Department of Neurology, Nantong First People's Hospital, Nantong, 226014, Jiangsu, China., Dong ZX; Department of Neurology, Nantong First People's Hospital, Nantong, 226014, Jiangsu, China., Zhu XY; Department of Neurology, Nantong First People's Hospital, Nantong, 226014, Jiangsu, China. ntyysuper123a@163.com.
Jazyk: angličtina
Zdroj: Biochemical genetics [Biochem Genet] 2024 Nov 21. Date of Electronic Publication: 2024 Nov 21.
DOI: 10.1007/s10528-024-10971-7
Abstrakt: Status epilepticus is a severe neurological emergency that often leads to long-term neuronal damage and functional impairment. Gastrodin is a compound widely used in traditional Chinese medicine with potential neuroprotective effects. This study aims to investigate the effects of GAS on neuroinflammation and injury caused by LiCl/pilocarpine-induced SE in young rats. SE in rats was induced using the LiCl/pilocarpine model. Morris water maze and Y-maze experiments were used for the behavioral test of rats. Enzyme-linked immunosorbent assay was utilized to quantify the levels of interleukin (IL)-1β, IL-6, and IL-8 levels, and biochemical kits assessed the levels of malondialdehyde, superoxide dismutase and glutathione peroxidase (GSH-px) in hippocampus tissues. Additionally, Western blot analysis was performed to evaluate the protein expression levels of p-p65, p65, p-IκBα and IκBα, which are key factors of the nuclear factor kappa B (NF-κB) signaling pathway. Compared to the control group, the SE group rats exhibited reduced learning and memory abilities. Markedly elevated levels of inflammatory factors (IL-1β, IL-6, and IL-8). The expression levels of p-p65 and p-IκBα were significantly upregulated, while IκBα levels were notably decreased. Following GAS treatment, the latency of seizure onset was significantly shortened, the incidence of SE was significantly reduced and the severity of nerve injury was alleviated. Additionally, both the inflammation levels and the oxidative stress were significantly decreased, primarily through inhibition NF-κB signaling pathway. These findings suggest that GAS may be a potential therapeutic agent for treating SE.
Competing Interests: Declarations. Conflict of interests: The authors declare no competing interests. Ethical Approval: All animal experiments were conducted in accordance with and this study has been approved by the Animal Ethics Committee of Nantong First People's Hospital and the Guide for the Care and Use of Laboratory Animals.
(© 2024. The Author(s).)
Databáze: MEDLINE
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