Glucosinolate Profiles of Capparis spp. and Maerua baillonii (Capparaceae) and Cytotoxicity of Methyl Isothiocyanate-Rich Isolates From Capparis spinosa subsp. rupestris.

Autor: Đulović A; Department of Organic Chemistry, Faculty of Chemistry and Technology, University of Split, Split, Croatia., Manase MJ; Faculté des Sciences, Université d'Antsiranana, Antsiranana, Madagascar., Čulić VČ; Department of Medical Chemistry and Biochemistry, School of Medicine, University of Split, Split, Croatia., Burčul F; Department of Analytical Chemistry, Faculty of Chemistry and Technology, University of Split, Split, Croatia., Rollin P; ICOA, Université d'Orléans et CNRS, Orléans, France., Blažević I; Department of Organic Chemistry, Faculty of Chemistry and Technology, University of Split, Split, Croatia.
Jazyk: angličtina
Zdroj: Chemistry & biodiversity [Chem Biodivers] 2024 Nov 21, pp. e202402573. Date of Electronic Publication: 2024 Nov 21.
DOI: 10.1002/cbdv.202402573
Abstrakt: This study investigates the glucosinolate profiles of various Capparis spp. and Maerua baillonii, from the Capparaceae family, and evaluates the cytotoxic potential of volatile isolates from Capparis spinosa subsp. rupestris. Using UHPLC-DAD-MS/MS, GSLs were identified and quantified across different plant tissues. Glucocapparin, predominant glucosinolate in C. spinosa subsp. spinosa, C. spinosa subsp. rupestris, and M. baillonii, were isolated and analyzed by NMR in its desulfo-form. Notably, glucosinolates were absent in C. richardii and specific tissues of M. baillonii (e.g., leaves and stems), underscoring species and/or tissue-specific variability within Capparaceae. Less common glucosinolates for Capparaceae plants, such as glucocochlearin, glucohirsutin, and glucoarabin were also detected. Methyl isothiocyanate was found to be the main volatile in all isolates obtained through various isolation methods, comprising ca. 80%-90% of the total volatiles. Methyl isothiocyanate-rich volatile isolates were tested for cytotoxicity against human breast cancer (MDA-MB-231) and bladder cancer (T24) cell lines using the MTT assay, showing significant activity with IC 50 values of 3.81 and 5.95 µg/mL, respectively. These findings underscore the anticancer potential of glucocapparin-bearing plants from the Capparaceae family.
(© 2024 Wiley‐VHCA AG, Zurich, Switzerland.)
Databáze: MEDLINE
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