Evaluation of the Immunoadjuvant Effects of miR-155-Chitosan Polyplex on Leishmania major Infected Mice.

Autor:	Pourabbasi Ardekan A; Department of Parasitology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Haghighi A; Department of Parasitology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Mohammadi-Yeganeh S; Medical Nanotechnology and Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.; Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Ghorbani-Bidkorpeh F; Department of Pharmaceutics and Pharmaceutical Nanotechnology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Kashefi S; Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Koochaki A; Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Movahedi S; Department of Pharmaceutics and Pharmaceutical Nanotechnology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Rahmani Y; Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Najafi Dastenaei A; Department of Immunology, Pasteur Institute of Iran, Tehran, Iran., Haji Molla Hoseini M; Medical Nanotechnology and Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.; Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Jazyk:	angličtina
Zdroj:	Immunological investigations [Immunol Invest] 2024 Nov 21, pp. 1-17. Date of Electronic Publication: 2024 Nov 21.
DOI:	10.1080/08820139.2024.2430695
Abstrakt:	Background: MicroRNAs have gained attention as key immunomodulators, with miR-155 specifically shown in various studies to drive macrophage polarization toward the classical phenotype. This polarization is crucial, as classical macrophages play a well-recognized role in differentiating type-1 immune responses and resisting Leishmania infection. Objective: The present study aims to evaluate the anti-leishmanial immunoadjuvant effects of the miR-155 chitosan polyplex (miR-155 CP). Methods: The anti-leishmanial immunoadjuvant activity of miR-155 CP synthesized by the coacervation method was assessed against L. major (MRHO/IR/75/ER) by analyzing the infectivity rate on RAW 264.7 cells in vitro.MiR-155 CP as an adjuvant co-administrated with soluble Leishmania antigen (SLA) for immunization of BALB/c mice, then the challenge was performed by subcutaneous injection of 1 × 10 6 L. major promastigotes. Eight weeks following the challenge, lesion size, parasite load, cytokine assay, and nitric oxide production were evaluated. Results: The nanoparticles were produced with a size of 233.87 ± 8 nm and a zeta potential of + 22.6 ± 2 mV with good transfection efficiency. The mean infection index among pretreated cells with miR-155 CP (72±1.1) decreased significantly compared to the control group (420 ± 2.8). The parasite burden and the size of the lesions were significantly reduced in the immunized infected mice. Vaccination by miR-155 CP/SLA triggered the production of IFN-γ and NO and changed the cytokine profile of antigen-specific cells.Conclusion:The effectiveness of the SLA vaccine can be enhanced by including miR-155 CP as an adjuvant. SLA and miR-155 CP co-administration improve the type-1 immune response. This enhanced immune response helps prevent severe leishmaniasis.
Databáze:	MEDLINE
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