A novel light-harvesting ZIF-9-TCPP as a promising FRET-based ratiometric fluorescence probe for sperm mobility.

Autor: Li YX; School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, P R China.; College of Chemical and Material Engineering, Quzhou University, Quzhou, Zhejiang 324000, P R China., Dai YX; School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, P R China.; College of Chemical and Material Engineering, Quzhou University, Quzhou, Zhejiang 324000, P R China., Wang JZ; School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, P R China., Chauvin J; University of Grenoble Alpes-CNRS, DCM UMR 5250, F-38000 Grenoble, France., Zhang XJ; School of Biomedical Engineering, Health Science Centre, Shenzhen University, Shenzhen 518060, P R China., Cosnier S; University of Grenoble Alpes-CNRS, DCM UMR 5250, F-38000 Grenoble, France.; Centre for Organic and Nanohybrid Electronics, Silesian University of Technology, Konarskiego 22B, 44-100 Gliwice, Poland.; Department of Physical Chemistry and Technology of Polymers, Silesian University of Technology, M. Strzody 9, 44-100 Gliwice, Poland., Marks RS; Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Beer-Sheva, 90089, Israel., Shan D; School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, P R China.
Jazyk: angličtina
Zdroj: The Analyst [Analyst] 2024 Nov 21. Date of Electronic Publication: 2024 Nov 21.
DOI: 10.1039/d4an01305k
Abstrakt: The concentration of zinc ions in semen is significantly correlated with sperm viability and male fertility. In this work, a reliable ratiometric fluorescence probe (ZIF-9-TCPP) based on the efficient Förster resonance energy transfer (FRET) process between two luminophores, benzimidazole (BIM) and meso -tetra (4-carboxyphenyl) porphyrin (TCPP) for Zn 2+ detection has been constructed, where the emissions of BIM and TCPP are used as reference and detection signals. The proximity of BIM and TCPP in one framework (ZIF-9-TCPP) and the overlapped spectra between BIM and TCPP afford the attainment of a highly efficient FRET (around 90% efficiency). Efficient FRET improves the fluorescence intensity of porphyrin to enhance the sensitivity of detection. The unique spectral shift resulting from Zn 2+ binding to the porphyrin ring ensures the selectivity of detection. In addition, the response mechanism of the proposed ratiometric probes to Zn 2+ has been investigated. This work provides a convenient way to design an efficient FRET system and a promising method for sperm mobility detection.
Databáze: MEDLINE
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