Genomic insights into heterotrophic nitrifying-aerobic denitrifying bacteria from petroleum terminal effluents.

Autor:	Medina LR; Department of Microbiology, Federal University of Viçosa, Viçosa, Minas Gerais, Brazil., Silva LCF; Department of Microbiology, Federal University of Viçosa, Viçosa, Minas Gerais, Brazil., Lima HS; Department of Microbiology, Federal University of Viçosa, Viçosa, Minas Gerais, Brazil., Vidigal PMP; Department of Biochemistry, Federal University of Viçosa, Viçosa, Minas Gerais, Brazil., de Castro AG; Department of Microbiology, Federal University of Viçosa, Viçosa, Minas Gerais, Brazil., de Paula Sousa M; Petrobras - Research and Development Center, Rio de Janeiro, RJ, Brazil., de Souza RS; Petrobras - Research and Development Center, Rio de Janeiro, RJ, Brazil., de Paula SO; Department of General Biology, Federal University of Viçosa, Viçosa, Minas Gerais, Brazil., da Silva CC; Department of Microbiology, Federal University of Viçosa, Viçosa, Minas Gerais, Brazil.
Jazyk:	angličtina
Zdroj:	Heliyon [Heliyon] 2024 Oct 18; Vol. 10 (21), pp. e39436. Date of Electronic Publication: 2024 Oct 18 (Print Publication: 2024).
DOI:	10.1016/j.heliyon.2024.e39436
Abstrakt:	Heterotrophic nitrification-aerobic denitrification (HN/AD) is a single-organism process that converts ammonia into nitrogen gas under strictly aerobic conditions, playing a crucial role in biological ammonia removal from industrial wastewater. Despite several studies, significant knowledge gaps remain about the genes involved in the process. This study aimed to characterize the genomes of four HN/AD bacterial strains, Pseudomonas stutzeri UFV5, Pseudomonas balearica UFV3, Rhodococcus ruber UFV2, and Gordonia amicalis UFV4, and identify potential genes involved in the HN/AD process. Results revealed that shared genes of these strains were primarily involved in amino acid and protein biosynthesis. The two Pseudomonas strains had more genes linked to nitrogen metabolism than the others. Additionally, four strains showed a significant number of hypothetical proteins and genes related to oxidative stress. Notably, no common nitrogen metabolism genes were found among the strains, indicating a lack of a shared HN/AD pathway. However, comparing these genomes with previous transcriptomic data of the P. stutzeri UFV5 identified nine shared proteins as potential HN/AD pathway candidates. This study enhances our understanding of the genomes of these HN/AD-capable bacterial strains and identifies nine candidate genes as markers for the HN/AD process. Competing Interests: The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:Lutecia Rigueira Medina reports financial support was provided by Minas Gerais State Foundation of Support to the Research. Cynthia Canedo da Silva reports a relationship with Petrobras that includes: funding grants. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2024 The Authors.)
Databáze:	MEDLINE
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