Fibrillarin homologs regulate translation in divergent cell lineages during planarian homeostasis and regeneration.

Autor:	Chen J; School of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China.; Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang, China.; Key Laboratory of Growth Regulation and Translational Research of Zhejiang Province, School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China.; Institute of Biology, Westlake Institute for Advanced Study, Hangzhou, Zhejiang, China., Li Y; Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang, China.; Key Laboratory of Growth Regulation and Translational Research of Zhejiang Province, School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China.; Institute of Biology, Westlake Institute for Advanced Study, Hangzhou, Zhejiang, China.; Fudan University, Shanghai, China., Wang Y; State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Vision Science, Guangzhou, China., Wang H; Center for Stem Cell and Regenerative Medicine and Bone Marrow Transplantation Center of the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310058, China.; Liangzhu Laboratory, Zhejiang University, 1369 West Wenyi Road, Hangzhou, 311121, China.; Institute of Hematology, Zhejiang University & Zhejiang Engineering Laboratory for Stem Cell and Immunotherapy, Hangzhou, 310058, China., Yang J; State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Vision Science, Guangzhou, China., Pan X; School of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China.; Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang, China.; Key Laboratory of Growth Regulation and Translational Research of Zhejiang Province, School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China.; Institute of Biology, Westlake Institute for Advanced Study, Hangzhou, Zhejiang, China., Zhao Y; Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang, China.; Key Laboratory of Growth Regulation and Translational Research of Zhejiang Province, School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China.; Institute of Biology, Westlake Institute for Advanced Study, Hangzhou, Zhejiang, China.; Fudan University, Shanghai, China., Xu H; School of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China.; Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang, China.; Key Laboratory of Growth Regulation and Translational Research of Zhejiang Province, School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China.; Institute of Biology, Westlake Institute for Advanced Study, Hangzhou, Zhejiang, China., Jiang P; Center for Stem Cell and Regenerative Medicine and Bone Marrow Transplantation Center of the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310058, China.; Liangzhu Laboratory, Zhejiang University, 1369 West Wenyi Road, Hangzhou, 311121, China.; Institute of Hematology, Zhejiang University & Zhejiang Engineering Laboratory for Stem Cell and Immunotherapy, Hangzhou, 310058, China., Qian P; Center for Stem Cell and Regenerative Medicine and Bone Marrow Transplantation Center of the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310058, China.; Liangzhu Laboratory, Zhejiang University, 1369 West Wenyi Road, Hangzhou, 311121, China.; Institute of Hematology, Zhejiang University & Zhejiang Engineering Laboratory for Stem Cell and Immunotherapy, Hangzhou, 310058, China., Wang H; State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Vision Science, Guangzhou, China., Xie Z; State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Vision Science, Guangzhou, China., Lei K; Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang, China. leikai@westlake.edu.cn.; Key Laboratory of Growth Regulation and Translational Research of Zhejiang Province, School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China. leikai@westlake.edu.cn.; Institute of Biology, Westlake Institute for Advanced Study, Hangzhou, Zhejiang, China. leikai@westlake.edu.cn.
Jazyk:	angličtina
Zdroj:	The EMBO journal [EMBO J] 2024 Dec; Vol. 43 (24), pp. 6591-6625. Date of Electronic Publication: 2024 Nov 20.
DOI:	10.1038/s44318-024-00315-x
Abstrakt:	Tissue homeostasis and regeneration involve complex cellular changes. The role of rRNA modification-dependent translational regulation in these processes remains largely unknown. Planarians, renowned for their ability to undergo remarkable tissue regeneration, provide an ideal model for the analysis of differential rRNA regulation in diverse cell types during tissue homeostasis and regeneration. We investigated the role of RNA 2'-O-methyltransferase, Fibrillarin (FBL), in the planarian Schmidtea mediterranea and identified two FBL homologs: Smed-fbl-1 (fbl-1) and Smed-fbl-2 (fbl-2). Both are essential for planarian regeneration, but play distinct roles: fbl-1 is crucial for progenitor cell differentiation, while fbl-2 is important for late-stage epidermal lineage specification. Different 2'-O-methylation patterns were observed upon fbl-1 and fbl-2 knockdown, suggesting their roles in translation of specific mRNA pools during regeneration. Ribo-seq analysis further revealed differing impacts of fbl-1 and fbl-2 knockdown on gene translation. These findings indicate divergent roles of the duplicate fbl genes in specific cell lineage development in planarians and suggest a role of rRNA modifications in translational regulation during tissue maintenance and regeneration. Competing Interests: Disclosure and competing interests statement. The authors declare no competing interests. (© 2024. The Author(s).)
Databáze:	MEDLINE
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