Discovery of novel and known viruses associated with toxigenic and non-toxigenic bloom forming diatoms from the Northern Adriatic Sea.

Autor: Turk Dermastia T; National Institute of Biology, Marine Biology Station Piran, Piran, Slovenia. Electronic address: timotej.turkdermastia@nib.si., Kutnjak D; National Institute of Biology, Departments of Biotechnology and Systems Biology, Ljubljana, Slovenia. Electronic address: denis.kutnjak@nib.si., Gutierrez-Aguirre I; National Institute of Biology, Departments of Biotechnology and Systems Biology, Ljubljana, Slovenia. Electronic address: ion.gutierrez@nib.si., Brussaard CPD; Royal Netherlands Institute of Sea Research (NIOZ), Department of Microbiology & Biogeochemistry, Texel, The Netherlands; Institute for Biodiversity and Ecosystem Dynamics (IBED), University of Amsterdam, 1098 Amsterdam, The Netherlands. Electronic address: corina.brussaard@nioz.nl., Bačnik K; National Institute of Biology, Departments of Biotechnology and Systems Biology, Ljubljana, Slovenia. Electronic address: katarina.bacnik@nib.si.
Jazyk: angličtina
Zdroj: Harmful algae [Harmful Algae] 2024 Nov; Vol. 139, pp. 102737. Date of Electronic Publication: 2024 Oct 15.
DOI: 10.1016/j.hal.2024.102737
Abstrakt: Algal blooms impact trophic interactions, community structure and element fluxes. Despite playing an important role in the demise of phytoplankton blooms, only few viruses infecting diatoms have been cultured. Pseudo-nitzschia is a widespread diatom genus that commonly blooms in coastal waters and contains toxin-producing species. This study describes the characterization of a novel virus infecting the toxigenic species Pseudo-nitzschia galaxiae isolated from the northern Adriatic Sea. The ssRNA virus PnGalRNAV has 29.5 nm ± 1.2 nm icosahedral virions and a genome size of 8.8 kb. It belongs to the picorna-like Marnaviridae family and shows high specificity for P. galaxiae infecting two genetically and morphologically distinct strains. We found two genetically distinct types of this virus and screening of the global virome database revealed matching sequences from the Mediterranean region and China, suggesting its global distribution. Another virus of similar shape and size infecting Pseudo-nitzschia calliantha was found, but its genome could not be determined. In addition, we have obtained and characterized a new virus that infects Chaetoceros tenuissimus. The replicase protein of this virus is very similar to the previously described ChTenDNAV type-II virus, but it has a unique genome and infection pattern. Our study is an important contribution to the collective diatom virus culture collection and will allow further investigation into how these viruses control diatom bloom termination, carbon export and toxin release in the case of Pseudo-nitzschia.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier B.V.)
Databáze: MEDLINE
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