Metagenomics and metaproteomics reveal the effects of sludge types and inoculation modes on N,N-dimethylformamide degradation pathways and the microbial community involved.

Autor: Xian Y; Guangxi Key Laboratory of Environmental Processes and Remediation in Ecologically Fragile Regions (Guangxi Normal University), 15 Yucai Road, 541004, PR China., Cao L; Guangxi Key Laboratory of Environmental Processes and Remediation in Ecologically Fragile Regions (Guangxi Normal University), 15 Yucai Road, 541004, PR China., Lu Y; Guangxi Key Laboratory of Environmental Processes and Remediation in Ecologically Fragile Regions (Guangxi Normal University), 15 Yucai Road, 541004, PR China; Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, 15 Yucai Road, Guilin 541004, PR China. Electronic address: lu_uyx@gxnu.edu.cn., Li Q; Guangxi Key Laboratory of Environmental Processes and Remediation in Ecologically Fragile Regions (Guangxi Normal University), 15 Yucai Road, 541004, PR China., Su C; Guangxi Key Laboratory of Environmental Processes and Remediation in Ecologically Fragile Regions (Guangxi Normal University), 15 Yucai Road, 541004, PR China; Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, 15 Yucai Road, Guilin 541004, PR China. Electronic address: suchengyuan2008@126.com., He Y; Guangxi Key Laboratory of Environmental Processes and Remediation in Ecologically Fragile Regions (Guangxi Normal University), 15 Yucai Road, 541004, PR China., Zhou G; Guangxi Key Laboratory of Environmental Processes and Remediation in Ecologically Fragile Regions (Guangxi Normal University), 15 Yucai Road, 541004, PR China., Chen S; Guangxi Key Laboratory of Environmental Processes and Remediation in Ecologically Fragile Regions (Guangxi Normal University), 15 Yucai Road, 541004, PR China., Gao S; Guangxi Key Laboratory of Environmental Processes and Remediation in Ecologically Fragile Regions (Guangxi Normal University), 15 Yucai Road, 541004, PR China.
Jazyk: angličtina
Zdroj: Journal of hazardous materials [J Hazard Mater] 2024 Nov 17; Vol. 481, pp. 136548. Date of Electronic Publication: 2024 Nov 17.
DOI: 10.1016/j.jhazmat.2024.136548
Abstrakt: This study demonstrated the effects of the sludge type and inoculation method on the N,N-dimethylformamide degradation pathway and associated microbial communities. The sludge type is critical for DMF metabolism, with acclimatized aerobic sludge having a significant advantage in terms of DMF metabolism performance, whereas acclimatized anaerobic sludge has a reduced DMF metabolism capacity. Metagenomic revealed increased abundances of Methanosarcina, Pelomona and Xanthobacter in the adapted anaerobic sludge, suggesting that anaerobic sludge can utilize the methyl products produced by DMF metabolism for growth. Adapted aerobic sludge had high Mycobacterium abundance, significantly boosting DMF hydrolysis. In addition, a large number of dmfA2 genes were found in aerobic sludge, more so in acclimatized sludge, indicating stronger DMF metabolism. Conversely, acclimatized anaerobic sludge showed lower abundance of dmd-tmd and mauA/B, qhpA genes, implying long-term DMF toxicity reduced anaerobic microbial activity. Metaproteomic analysis showed that Methanosarcina and Methanomethylovorans enzymes in anaerobic sludge metabolized dimethylamine and methylamine to methane, aiding DMF degradation. In the aerobic sludge, aminohydrolase proteins, which hydrolyze DMF, were significantly upregulated. These findings provide insights into DMF wastewater treatment.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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