Application of 1-octanol in the extraction and GC-FID analysis of volatile organic compounds produced in biogas and biohydrogen processes.

Autor: Pavini WD; São Paulo State University (UNESP), Institute of Chemistry, Center for Monitoring and Research of the Quality of Fuels, Biofuels, Crude Oil, and Derivatives (CEMPEQC), Rua Professor Francisco Degni, 55, Araraquara, SP 14800-900, Brazil., Ferreira JVS; Federal University of Paraná (UFPR), Palotina campus, Rua Pioneiro, 2153, Palotina, PR 85950-000, Brazil., Flumignan DL; São Paulo State University (UNESP), Institute of Chemistry, Center for Monitoring and Research of the Quality of Fuels, Biofuels, Crude Oil, and Derivatives (CEMPEQC), Rua Professor Francisco Degni, 55, Araraquara, SP 14800-900, Brazil; São Paulo Federal Institute of Education, Science and Technology (IFSP), Matão campus, Rua Stéfano D'Avassi, 625, Matão, SP 15991-502, Brazil., Maintinguer SI; São Paulo State University (UNESP), Institute of Chemistry, Center for Monitoring and Research of the Quality of Fuels, Biofuels, Crude Oil, and Derivatives (CEMPEQC), Rua Professor Francisco Degni, 55, Araraquara, SP 14800-900, Brazil., Oliveira JE; São Paulo State University (UNESP), Institute of Chemistry, Center for Monitoring and Research of the Quality of Fuels, Biofuels, Crude Oil, and Derivatives (CEMPEQC), Rua Professor Francisco Degni, 55, Araraquara, SP 14800-900, Brazil., Sequinel R; São Paulo State University (UNESP), Institute of Chemistry, Center for Monitoring and Research of the Quality of Fuels, Biofuels, Crude Oil, and Derivatives (CEMPEQC), Rua Professor Francisco Degni, 55, Araraquara, SP 14800-900, Brazil; Federal University of Paraná (UFPR), Palotina campus, Rua Pioneiro, 2153, Palotina, PR 85950-000, Brazil. Electronic address: rodrigosequinel@ufpr.br.
Jazyk: angličtina
Zdroj: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [J Chromatogr B Analyt Technol Biomed Life Sci] 2024 Nov 17; Vol. 1250, pp. 124376. Date of Electronic Publication: 2024 Nov 17.
DOI: 10.1016/j.jchromb.2024.124376
Abstrakt: Information about the volatile organic compounds generated in biogas and hydrogen production bioreactors is essential to elucidate the metabolic routes and varying yields of CH 4 and H 2 processes. In this work, the determination of 12 compounds (acetone, methanol, ethanol, 1-propanol, 1-butanol, and acetic, propanoic, butyric, isovaleric, valeric, caproic, and lactic acids) was performed by gas chromatography, after a vortex-assisted liquid-liquid microextraction (VALLME) procedure using 1.2 mL of crude sample and 400 µL of 1-octanol. Optimization of the separation process was performed, considering the solvent viscosity. The analytical curves were validated using ANOVA, demonstrating satisfactory precision and accuracy. Selectivity was confirmed by GC-MS analysis, which allowed the detection of glycerol, 1,3-propanediol, and 1,3-butanediol in some samples. Methanol levels exceeded the upper limit of quantification, with acetic acid and ethanol being the predominant compounds in the analyzed reactors. An additional investigation was conducted to assess potential interferences for lactic acid. The developed method employs a biodegradable extraction solvent, without any need for a dispersing solvent, and involves a single chromatographic run, without any derivatization steps.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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