Strong Correlation Between Double-Strand DNA Breaks and Total Sperm DNA Fragmentation in the Human Ejaculate.

Autor: Gosálvez J; Department of Biology, Universidad Autónoma de Madrid, Madrid, Spain. Electronic address: jaime.gosalvez@uam.es., Johnston SD; School of Environment, University of Queensland, Gatton, Queensland, Australia; School of Veterinary Science, University of Queensland, Gatton, Queensland, Australia; Halotech DNA, Calle Faraday SN, Parque Científico de Madrid, Madrid, Spain., Prado A; School of Veterinary Science, University of Queensland, Gatton, Queensland, Australia; Halotech DNA, Calle Faraday SN, Parque Científico de Madrid, Madrid, Spain., López-Fernández C; Department of Biology, Universidad Autónoma de Madrid, Madrid, Spain., Contreras P; School of Veterinary Science, University of Queensland, Gatton, Queensland, Australia; Halotech DNA, Calle Faraday SN, Parque Científico de Madrid, Madrid, Spain., Bartolomé-Nebreda J; Department of Biology, Universidad Autónoma de Madrid, Madrid, Spain; School of Veterinary Science, University of Queensland, Gatton, Queensland, Australia; Halotech DNA, Calle Faraday SN, Parque Científico de Madrid, Madrid, Spain., González-Martínez M; GINEMED Clinic, Farmacéutico Murillo Herrera, Sevilla, Spain., Fernández JL; Unidad de Genética, Complejo Hospitalario Universitario A Coruña, Instituto de Investigación Biomédica de A Coruña, A Coruña, Spain; Laboratorio de Genética Molecular y Radiobiología, Centro Oncológico de Galicia. A Coruña, Spain., de la Vega CG; Department of Biology, Universidad Autónoma de Madrid, Madrid, Spain., Góngora A; Centro de Fertilidad, Mexico City, Mexico.
Jazyk: angličtina
Zdroj: Archives of medical research [Arch Med Res] 2024 Dec; Vol. 55 (8), pp. 103122. Date of Electronic Publication: 2024 Nov 20.
DOI: 10.1016/j.arcmed.2024.103122
Abstrakt: Background: Double- and single-strand DNA breaks (DSBs and SSBs, respectively) in spermatozoa, which emerge from intrinsic and extrinsic degenerative processes, are likely related to the underlying male pathology.
Aim: To determine whether the incidence of DSBs in the human ejaculate is a consistent predictor of whole sperm DNA fragmentation (W-SDF = SSBs + DSBs).
Methods: A correlation between the proportion of spermatozoa that showed whole W-SDF and those displaying only DSBs in DNA. Two patient cohorts were established: W-SDF ≤30% (low SDF; n = 153) and W-SDF ≥30% (high SDF; n = 222).
Results: An increasing level of W-SDF is associated with an increased incidence of DSBs in the ejaculate. When data from both the low and high W-SDF groups were combined, a linear relationship was observed, with DSBs increasing by 0.799 units for each unit increase in W-SDF. However, when the cohorts were analyzed separately, the relationships differed. In the low SDF group, DSBs increased linearly by 0.559 units for each unit increase in W-SDF. In the high SDF group, DSBs increased exponentially by 0.602 units per unit of W-SDF. Furthermore, the data dispersion between the two variables was significantly different between the cohorts, with the high SDF group showing 0.8 times greater variability than the low SDF group.
Conclusions: While the presence of DSBs in sperm is correlated with the W-SDF present in raw semen samples, the biological mechanisms responsible for DSBs are expressed in different proportions and/or at different levels in ejaculates with higher levels of DNA damage.
(Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE