Immunocytochemistry-Based Detection of FOXO Isoforms in Human Cancer and Fibroblasts.

Autor:	Jimenez L; Sols-Morreale Biomedical Research Institute (IIBM), Spanish National Research Council (CSIC), Universidad Autónoma de Madrid (UAM), Madrid, Spain., Domínguez L; Sols-Morreale Biomedical Research Institute (IIBM), Spanish National Research Council (CSIC), Universidad Autónoma de Madrid (UAM), Madrid, Spain., Amenabar C; Sols-Morreale Biomedical Research Institute (IIBM), Spanish National Research Council (CSIC), Universidad Autónoma de Madrid (UAM), Madrid, Spain., Serrão G; ABC-RI, Algarve Biomedical Center Research Institute, Algarve Biomedical Center, Faro, Portugal.; Faculty of Medicine and Biomedical Sciences, University of Algarve, Faro, Portugal., Link W; Department of Cancer Biology, Sols-Morreale Biomedical Research Institute (IIBM), Spanish National Research Council (CSIC), Universidad Autónoma de Madrid (UAM), Madrid, Spain., Ferreira BI; ABC-RI, Algarve Biomedical Center Research Institute, Algarve Biomedical Center, Faro, Portugal. biferreira@ualg.pt.; Faculty of Medicine and Biomedical Sciences, University of Algarve, Faro, Portugal. biferreira@ualg.pt., Santos B; ABC-RI, Algarve Biomedical Center Research Institute, Algarve Biomedical Center, Faro, Portugal. a66853@ualg.pt.; Faculty of Medicine and Biomedical Sciences, University of Algarve, Faro, Portugal. a66853@ualg.pt.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2025; Vol. 2871, pp. 171-176.
DOI:	10.1007/978-1-0716-4217-7_16
Abstrakt:	In mammals, the FOXO protein family consists of four distinct isoforms: FOXO1, FOXO3, FOXO4, and FOXO6. These isoforms are key players in a wide spectrum of physiological and pathological processes, including context-specific tumor suppression. FOXO3, in particular, has emerged as a gene associated with extraordinary human longevity. While these four FOXO isoforms share common biological functions, the mechanisms underlying their overlapping and distinct roles remain less understood. It is believed that intrinsic properties and context-dependent factors contribute to isoform-specific and nonredundant FOXO functions. One promising avenue for unraveling the commonalities and specificities of these proteins involves characterizing their expression patterns in specific cell types and their activation in response to different stimuli. To facilitate this, we have developed immunocytochemistry methods capable of detecting FOXO isoforms in a highly specific manner within various human cancer cell types and fibroblasts. Importantly, this approach enables the visualization of endogenous FOXO proteins as they translocate into the cell nucleus in response to different stimuli. In this article, we present a comprehensive guide to these procedures, offering valuable insights into the distinct roles of FOXO isoforms in cellular function. (© 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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