Cardiomyocyte-Specific FoxO1 Knockout Mice as Tools to Assess Cardiac Hypertrophy and Key Experimental Considerations Using Cre-loxP.
| Autor: | Weeks KL; Department of Anatomy and Physiology, The University of Melbourne, Parkville, VIC, Australia. Kate.weeks@unimelb.edu.au.; Baker Department of Cardiometabolic Health, The University of Melbourne, Parkville, VIC, Australia. Kate.weeks@unimelb.edu.au.; Central Clinical School, Monash University, Clayton, VIC, Australia. Kate.weeks@unimelb.edu.au., McMullen JR; Baker Department of Cardiometabolic Health, The University of Melbourne, Parkville, VIC, Australia. Julie.mcmullen@hri.org.au.; Baker Heart and Diabetes Institute, Melbourne, VIC, Australia. Julie.mcmullen@hri.org.au.; Baker Department of Cardiovascular Research, Translation and Implementation, La Trobe University, Melbourne, VIC, Australia. Julie.mcmullen@hri.org.au.; Monash Alfred Baker Centre for Cardiovascular Research, Faculty of Medicine, Nursing and Health Sciences, Monash University, Melbourne, VIC, Australia. Julie.mcmullen@hri.org.au.; Heart Research Institute, Sydney, N.S.W., Australia. Julie.mcmullen@hri.org.au. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2025; Vol. 2871, pp. 69-81. |
| DOI: | 10.1007/978-1-0716-4217-7_7 |
| Abstrakt: | The generation of tissue-specific mouse models has provided a powerful strategy to understand the role of genes in specific tissues/cells of interest under control/basal conditions and in response to physiological and pathological stimuli. Here we describe the generation of cardiomyocyte-specific FoxO1 knockout mice using Cre-loxP technology to examine the role of FoxO1 for the induction of heart enlargement (cardiac hypertrophy) in settings of health and disease. We highlight breeding strategies for generating tissue-specific mouse models and key experimental considerations during characterization. (© 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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