Whole genome sequencing revealed high proportions of ST152 MRSA among clinical Staphylococcus aureus isolates from ten hospitals in Ghana.

Autor:	Egyir B; Department of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra, Ghana., Owusu-Nyantakyi C; Department of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra, Ghana., Bortey A; Department of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra, Ghana., Rabbi Amuasi G; Department of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra, Ghana., Owusu FA; Department of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra, Ghana., Boateng W; Department of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra, Ghana., Ahmed H; Department of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra, Ghana., Danso JK; Department of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra, Ghana., Oclu AAG; Department of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra, Ghana., Mohktar Q; Department of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra, Ghana., Tetteh-Ocloo G; Eastern Regional Hospital, Koforidua, Ghana., Amegbletor H; St. Martin de Porress Hospital, Eikwe, Ghana., Fosu K; Bolgatanga Regional Hospital, Bolgatanga, Ghana., Tetteh FKM; 37 Military Hospital, Accra, Ghana., Asante-Sefa S; Sekondi Public Health Laboratory, Effia Nkwanta Regional Hospital, Takoradi, Ghana., Deberu ON; Tamale Teaching Hospital, Tamale, Ghana., Osei KM; Tamale Teaching Hospital, Tamale, Ghana., Twasam J; Lekma Hospital, Accra, Ghana., Kodom S; University of Ghana Hospital, Legon, Ghana., Gyinae E; Korle bu Teaching Hospital, Accra, Ghana., Sampah J; St. Patrick's Hospital, Ofinso, Ghana., Dzifa Dayie N; Department of Medical Microbiology, University of Ghana Medical School, Korle-Bu, Ghana., Obeng-Nkrumah N; Department of Medical Laboratory Sciences, School of Biomedical and Allied Health Sciences, University of Ghana, Accra, Ghana., Mills-Pappoe WA; Clinical Laboratory Unit, Institutional Care Division-Ghana Health Service, Accra, Ghana., Boateng G; Public Health Reference Lab, Korle-Bu, Ghana., Nilsson P; Research Group for Global Capacity Building, National Food Institute, WHO Collaborating Centre (WHO CC) for Antimicrobial Resistance in Foodborne Pathogens and Genomics, FAO Reference Laboratory (FAO RL) for Antimicrobial Resistance, European Union Reference Laboratory for Antimicrobial Resistance (EURL-AR), Technical University of Denmark, Kongens Lyngby, Denmark., Bonful HA; Department of Epidemiology and Disease Control, School of Public Health, University of Ghana, Accra, Ghana., Adu B; Department of Immunology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra, Ghana., Hendriksen RS; Research Group for Global Capacity Building, National Food Institute, WHO Collaborating Centre (WHO CC) for Antimicrobial Resistance in Foodborne Pathogens and Genomics, FAO Reference Laboratory (FAO RL) for Antimicrobial Resistance, European Union Reference Laboratory for Antimicrobial Resistance (EURL-AR), Technical University of Denmark, Kongens Lyngby, Denmark.
Jazyk:	angličtina
Zdroj:	MSphere [mSphere] 2024 Dec 19; Vol. 9 (12), pp. e0044624. Date of Electronic Publication: 2024 Nov 20.
DOI:	10.1128/msphere.00446-24
Abstrakt:	Previous studies in Ghana indicated low prevalence of methicillin-resistant Staphylococcus aureus (MRSA) and predominance of ST152 methicillin-susceptible S. aureus (MSSA) among clinical isolates. ST152 MRSA clones are associated with severe infections and epidemics. Using whole genome sequencing (WGS), 159 S . aureus isolated from clinical sources (wound, blood, urine, ear, abscess, umbilical cord, eye, vaginal samples, and others) from 10 hospitals across Ghana were investigated. mecA (gene for methicillin resistance) was detected in 38% of the isolates. Panton-Valentine leucocidin toxin (PVL) gene occurred in 65% isolates, with 84% of the MRSA's harboring the PVL gene. ST152 was the major clone, with 74% harboring the mecA gene. Other MRSA clones detected were ST5, ST5204, ST852, and ST1. MSSA clones included ST3249, ST152, ST5, ST1, and ST8. Twenty-three genes encoding resistance to 12 antimicrobial classes were observed with blaZ (97%) being the most prevalent. Other predominant resistance genes included tetK (46%), cat (42%), and dfrG (36%) encoding resistance for tetracyclines, phenicols, and diaminopyrimidine, respectively. Virulence genes for enterotoxins, biofilms, toxic-shock-syndrome toxins, hemolysins , and leukotoxins were also detected. Phylogenetic analysis revealed a shift in the dominant clone from MSSA ST152 to MRSA ST152 over the past decade. The study provides valuable insights into the genomic content of S. aureus from clinical sources in Ghana. The finding of ST152 MRSA in high numbers suggests a shifting epidemiological landscape of these pathogens and continuous surveillance using robust tools like WGS is needed to monitor the rise and spread of these epidemic clones in the country.IMPORTANCESince its emergence in 1959, MRSA has been a significant public health concern, causing infections in both clinical and community settings. Patients with MRSA-related infections experience higher mortality rates due to its ability to evade antimicrobials and immune defenses. In Ghana, understanding the molecular epidemiology of MRSA has been hindered by the lack of appropriate laboratory infrastructure and the limited capacity for molecular data analysis. This study, the largest genomic study of S. aureus in Ghana, addresses this gap by utilizing whole genome sequencing to examine the diversity of circulating S. aureus strains from 10 hospitals. Our findings highlight the predominance of pandemic clones, particularly ST152, and the notable transition of ST152 MSSA to ST152 MRSA over the past decade. The findings from this study supports AMR surveillance efforts in Ghana and emphasize the importance of implementing genomic surveillance using WGS to comprehensively monitor the rise and spread of multi-drug-resitant organisms such as MRSA in the country. Competing Interests: The authors declare no conflict of interest.
Databáze:	MEDLINE
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