Staphylococcus aureus can use an alternative pathway to be internalized by osteoblasts in absence of β1 integrins.

Autor: Tricou LP; Centre International de Recherche en Infectiologie (CIRI), Université de Lyon, CNRS, UMR5308, ENS de Lyon, Inserm, U1111, Université Claude Bernard Lyon 1, Lyon, France.; ISPB Faculté de Pharmacie, Université Claude Bernard Lyon 1, Lyon, France.; Department of Orthopaedic Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, 02215, USA., Mouton W; Centre International de Recherche en Infectiologie (CIRI), Université de Lyon, CNRS, UMR5308, ENS de Lyon, Inserm, U1111, Université Claude Bernard Lyon 1, Lyon, France.; Joint Research Unit Civils Hospices of Lyon-bioMérieux, Hospices Civils de Lyon, Lyon Sud Hospital, Pierre-Bénite, France., Cara A; Centre International de Recherche en Infectiologie (CIRI), Université de Lyon, CNRS, UMR5308, ENS de Lyon, Inserm, U1111, Université Claude Bernard Lyon 1, Lyon, France., Trouillet-Assant S; Centre International de Recherche en Infectiologie (CIRI), Université de Lyon, CNRS, UMR5308, ENS de Lyon, Inserm, U1111, Université Claude Bernard Lyon 1, Lyon, France.; Joint Research Unit Civils Hospices of Lyon-bioMérieux, Hospices Civils de Lyon, Lyon Sud Hospital, Pierre-Bénite, France., Bouvard D; Centre de Recherche en Biologie Cellulaire de Montpellier (CRBM), CNRS UMR 5237, Université de Montpellier, Montpellier, France., Laurent F; Centre International de Recherche en Infectiologie (CIRI), Université de Lyon, CNRS, UMR5308, ENS de Lyon, Inserm, U1111, Université Claude Bernard Lyon 1, Lyon, France.; ISPB Faculté de Pharmacie, Université Claude Bernard Lyon 1, Lyon, France.; Centre National de Référence des Staphylocoques, Institut des Agents Infectieux, Hospices Civils de Lyon, Lyon, France.; Centre Interrégional de Référence pour la Prise en Charge des Infections Ostéo-Articulaires Complexes (CRIOAc Lyon), Hospices Civils de Lyon, Lyon, France., Diot A; Centre International de Recherche en Infectiologie (CIRI), Université de Lyon, CNRS, UMR5308, ENS de Lyon, Inserm, U1111, Université Claude Bernard Lyon 1, Lyon, France., Josse J; Centre International de Recherche en Infectiologie (CIRI), Université de Lyon, CNRS, UMR5308, ENS de Lyon, Inserm, U1111, Université Claude Bernard Lyon 1, Lyon, France. jerome.josse@univ-lyon1.fr.; ISPB Faculté de Pharmacie, Université Claude Bernard Lyon 1, Lyon, France. jerome.josse@univ-lyon1.fr.; Centre Interrégional de Référence pour la Prise en Charge des Infections Ostéo-Articulaires Complexes (CRIOAc Lyon), Hospices Civils de Lyon, Lyon, France. jerome.josse@univ-lyon1.fr.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Nov 19; Vol. 14 (1), pp. 28643. Date of Electronic Publication: 2024 Nov 19.
DOI: 10.1038/s41598-024-78754-z
Abstrakt: Staphylococcus aureus main internalization mechanism in osteoblasts relies on a tripartite interaction between bacterial fibronectin-binding proteins, extracellular matrix soluble fibronectin, and osteoblasts' β1 integrins. Caveolins, and particularly caveolin-1, have been shown to limit the plasma membrane microdomain mobility, and consequently reduce the uptake of S. aureus in keratinocytes. In this study, we aimed to deepen our understanding of the molecular mechanisms underlying S. aureus internalization in osteoblasts. Mechanistically, S. aureus internalization requires endosomal recycling of β1 integrins as well as downstream effectors such as Src, Rac1, and PAK1. Surprisingly, in β1 integrin deficient osteoblasts, S. aureus internalization is restored when Caveolin-1 is absent and requires αvβ3/5 integrins as backup fibronectin receptors. Altogether, our data support that β1 integrins regulate the level of detergent-resistant membrane at the plasma membrane in a an endosomal and Caveolin-1 dependent manner.
Competing Interests: Declarations Competing interests The authors declare no competing interests.
(© 2024. The Author(s).)
Databáze: MEDLINE
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