A new cancer/testis long noncoding RNA, the OTP-AS1 RNA.

Autor: Karnaukhova IK; Vavilov Institute of General Genetics, Moscow, Russia.; The Biomedical Center, St. Petersburg, Russia., Polev DE; The Biomedical Center, St. Petersburg, Russia.; Saint-Petersburg Pasteur Institute, St. Petersburg, Russia., Krukovskaya LL; The Biomedical Center, St. Petersburg, Russia., Makashov AA; Peter the Great St. Petersburg Polytechnic University, St. Petersburg, Russia., Masharsky AE; The Biomedical Center, St. Petersburg, Russia., Nazarenko OV; The Biomedical Center, St. Petersburg, Russia., Poverennaya IV; Vavilov Institute of General Genetics, Moscow, Russia.; Center for Brain Research, Department of Neuroimmunology, Medical University Vienna, Vienna, Austria., Makeev VJ; Vavilov Institute of General Genetics, Moscow, Russia.; Moscow Center for Advanced Studies, Moscow, Russia.; Cancer Research UK National Biomarker Centre University of Manchester, Manchester, M20 4BX, UK., Akulova EB; Vavilov Institute of General Genetics, Moscow, Russia., Kozlov AP; Vavilov Institute of General Genetics, Moscow, Russia. contact@biomed.spb.ru.; The Biomedical Center, St. Petersburg, Russia. contact@biomed.spb.ru.; Peter the Great St. Petersburg Polytechnic University, St. Petersburg, Russia. contact@biomed.spb.ru.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Nov 19; Vol. 14 (1), pp. 28676. Date of Electronic Publication: 2024 Nov 19.
DOI: 10.1038/s41598-024-80065-2
Abstrakt: The orthopedia homeobox (OTP) gene encodes a homeodomain-containing transcription factor involved in brain development. OTP is mapped to human chromosome 5q14.1. Earlier we described transcription in the second intron of this gene in wide variety of tumors, but among normal tissues only in testis. In GeneBank these transcripts are represented by several 300-400 nucleotide long AI267901-like ESTs. We assumed that the AI267901-like ESTs belonged to the longer transcript(s). We used the Rapid Amplification of cDNA Ends (RACE) approach and other methods to find the full-length transcript. The transcript we found was a 2436 nucleotide polyadenylated sequence in antisense to OTP gene. The corresponding gene consisted of two exons separated by an intron of 2961 bp. The first exon was found to be 91 bp long and located in the third exon of OTP. The second exon was 2345 bp long and located in the second intron of OTP. We have shown the expression of this gene in many human tumors but as few as a single sample of normal testis. The transcript lacked significant ORFs suggesting that we discovered a new antisense cancer/testis (CT) sequence OTP-AS1 (OTP-antisense RNA 1), which belongs to the class of long noncoding RNAs (lncRNAs). According to our findings we assume that OTP-AS1 and OTP genes may be a CT-coding gene/CT-ncRNA pair, or sense-antisense gene pair involved in regulatory interactions.
Competing Interests: Competing interests The authors declare no competing interests.
(© 2024. The Author(s).)
Databáze: MEDLINE
Externí odkaz: