Development of selective ssDNA micro-probe for PD1 detection as a novel strategy for cancer imaging.

Autor: Malicki S; Laboratory of Proteolysis and Post-translational Modification of Proteins, Malopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7a, Krakow, 30-387, Poland. stanislaw.malicki@uj.edu.pl.; Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, Krakow, 30-387, Poland. stanislaw.malicki@uj.edu.pl., Czarna A; Protein Crystallography Research, Group Malopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7a, Krakow, 30-387, Poland., Żyła E; Protein Crystallography Research, Group Malopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7a, Krakow, 30-387, Poland.; Department of Cell Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, Krakow, 30-387, Poland., Pucelik B; Protein Crystallography Research, Group Malopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7a, Krakow, 30-387, Poland.; 5Łukasiewicz Research Network, Krakow Institute of Technology, ul. Zakopiańska 73, Kraków, 30-418, Poland., Gałan W; Department of Computational Biophysics and Bioinformatics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, Krakow, 30-387, Poland., Chruścicka B; Laboratory of Proteolysis and Post-translational Modification of Proteins, Malopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7a, Krakow, 30-387, Poland.; Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, Krakow, 30-387, Poland., Kamińska M; Broegelmann Research Laboratory, University of Bergen, Haukeland universitetssykehus Laboratoriebygget, Bergen, 5009, Norway., Sochaj-Gregorczyk A; Laboratory of Proteolysis and Post-translational Modification of Proteins, Malopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7a, Krakow, 30-387, Poland.; Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, Krakow, 30-387, Poland., Magiera-Mularz K; Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, Krakow, 30-387, Poland.; Laboratory of protein NMR, Malopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7a, Krakow, 30- 387, Poland., Wang J; School of Biomedical Sciences and Engineering, Guangzhou International Campus, South China University of Technology, Guangzhou, 511442, People's Republic of China., Winiarski M; 2nd Department of General Surgery, Faculty of Medicine, Jagiellonian University Medical College, Kraków, 31-008, Poland., Benedyk-Machaczka M; Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, Krakow, 30-387, Poland., Kozieł J; Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, Krakow, 30-387, Poland., Dubin G; Protein Crystallography Research, Group Malopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7a, Krakow, 30-387, Poland. grzegorz.dubin@uj.edu.pl., Mydel P; Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, Krakow, 30-387, Poland. piotr.mydel@uib.no.; Broegelmann Research Laboratory, University of Bergen, Haukeland universitetssykehus Laboratoriebygget, Bergen, 5009, Norway. piotr.mydel@uib.no.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Nov 19; Vol. 14 (1), pp. 28652. Date of Electronic Publication: 2024 Nov 19.
DOI: 10.1038/s41598-024-74891-7
Abstrakt: Programmed death receptor 1, PD1, modulates the function of immune cells by providing inhibitory signals and constitutes the marker of immune exhaustion. Monitoring the level of PD1 promises a useful diagnostic approach in autoimmune diseases and cancer. Here we describe the development of an ssDNA aptamer-based molecular probe capable of specific recognition of human PD1 receptor. The aptamer was selected using SELEX, its sequence was further optimized, and the affinity and specificity were determined in biochemical assays. The aptamer was converted into a fluorescent probe and its potential in molecular imaging was demonstrated in a culture of human cells overexpressing PD1 and murine pancreatic organoids / immune cells mixed co-culture model. We conclude that the provided aptamers are suitable probes for imaging of PD1 expressing immune cells even in complex cellular models and may find future utility as diagnostic tools.
Competing Interests: Declarations Competing interests The authors declare no competing interests. Ethics approval All animal experiments were approved by the Animal Care and Use Committee at SCUT. The study was carried out in compliance with the Animal Research: Reporting of In Vivo Experiments (ARRIVE) guidelines. And all methods were carried out in accordance with relevant guidelines and regulations.
(© 2024. The Author(s).)
Databáze: MEDLINE
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