Glycyrrhizic Acid Formulated in Hydrotalcite Nanocarriers Intended to Act as a Hepatoprotective Agent.

Autor: Hernández M; Laboratorio de Investigación y Posgrado en Tecnología Farmacéutica (L-322), Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Av. 1 de Mayo S/N, Cuautitlán Izcalli, CP 54740, Edo. de Méx, México., Lima E; Laboratorio de Fisicoquímica y Reactividad de Superficies (LaFReS), Instituto de Investigaciones en Materiales, Universidad Nacional Autónoma de México, Circuito Exterior S/N, Cd. Universitaria, Del. Coyoacán, CP 04510, Ciudad de Mexico, México., Magaña JJ; Laboratorio de Medicina Genómica, Departamento de Genética, Instituto Nacional de Rehabilitación, Calz. México Xochimilco N° 289, CP 14389, Ciudad de Mexico, Mexico.; Departamento de Bioingeniería, Escuela de Ingeniería y Ciencias, Tecnológico de Monterrey, Campus Ciudad de México, CP 14380, Ciudad de México, México., Ganem-Rondero A; Laboratorio de Investigación y Posgrado en Tecnología Farmacéutica (L-322), Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Av. 1 de Mayo S/N, Cuautitlán Izcalli, CP 54740, Edo. de Méx, México. ganemq@hotmail.com.
Jazyk: angličtina
Zdroj: The AAPS journal [AAPS J] 2024 Nov 19; Vol. 27 (1), pp. 2. Date of Electronic Publication: 2024 Nov 19.
DOI: 10.1208/s12248-024-00986-8
Abstrakt: The article focuses on preparing a nanoformulation based on hydrotalcite and glycyrrhizic acid (GA), seeking a hepatoprotective effect. For this purpose, hydrotalcite-GA formulations were prepared by varying the following conditions to obtain optimal systems in terms of size and PDI (the lowest values), and Z potential (the highest values): (i) type of hydrotalcite (obtained by co-precipitation or calcined hydrotalcite); method used (ultrasound or high shear stirring), and (iii) type of stabilizer (Tween®80 or Pluronic® F-127). The best results were obtained using hydrotalcite obtained by co-precipitation, with high shear stirring and adding a stabilizer, either Tween®80 (HT-T80-GA: mean particle size = 315 nm, PDI = 0.18, Z potential = -20.93) or Pluronic® F-127 (HT-PF127-GA: mean particle size = 307 nm; PDI = 0.27, Z potential = -21.03). After stability studies, the HT-T80-GA formulation was chosen to study antioxidant activity, cytotoxicity, and intracellular penetration capacity. Although the hepatoprotective effect of GA in solution allowed a high viability and antioxidant activity, the fact of including GA in the HT-T80-GA formulation favored its penetration into hepatocytes, with a decrease in Caspase-3/7 expression of C-9 hepatocyte cells treated with H 2 O 2 , suggesting the capacity to inhibit apoptosis.
Competing Interests: Declarations Ethics Approval and Consent to Participate Not applicable. Human and Animal Rights No animals/humans were used for studies that are the basis of this research. Conflict of Interest No potential conflict of interest was reported by the authors.
(© 2024. The Author(s), under exclusive licence to American Association of Pharmaceutical Scientists.)
Databáze: MEDLINE
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