ZBTB24 is a conserved multifaceted transcription factor at genes and centromeres that governs the DNA methylation state and expression of satellite repeats.

Autor:	Grillo G; UMR7216 Epigénétique et Destin Cellulaire, CNRS, Université de Paris Cité, Epigenetics and Cell Fate, Lamarck building, 35 rue Hélène Brion, Paris F-75013, France., Boyarchuk E; UMR7216 Epigénétique et Destin Cellulaire, CNRS, Université de Paris Cité, Epigenetics and Cell Fate, Lamarck building, 35 rue Hélène Brion, Paris F-75013, France.; UMR7216, Genome engineering in epigenetics platform (GENIE), Lamarck building, 35 rue Hélène Brion, Paris F-75013, France., Mihic S; UMR7216 Epigénétique et Destin Cellulaire, CNRS, Université de Paris Cité, Epigenetics and Cell Fate, Lamarck building, 35 rue Hélène Brion, Paris F-75013, France., Ivkovic I; UMR7216 Epigénétique et Destin Cellulaire, CNRS, Université de Paris Cité, Epigenetics and Cell Fate, Lamarck building, 35 rue Hélène Brion, Paris F-75013, France., Bertrand M; Bioinformatics and Biostatistics Core Facility, iCONICS, Institut du Cerveau (ICM), Sorbonne Université, INSERM, CNRS, Hôpital Pitié-Salpêtrière, 47 bd de l'hôpital, Paris F-75013, France., Jouneau A; Université Paris-Saclay, UVSQ, INRAE, BREED, Bâtiment 230, Domaine de Vilvert, Jouy-en-Josas 78350, France.; Ecole Nationale Vétérinaire d'Alfort, BREED, 7 av. du Général de Gaulle, Maisons-Alfort 94700, France., Dahlet T; University of Strasbourg, 4 rue Blaise Pascal, Strasbourg 67081, France.; CNRS UMR7242, Biotechnology and Cell Signaling, 300 bd Sébastien Brant, Illkirch 67412, France., Dumas M; University of Strasbourg, 4 rue Blaise Pascal, Strasbourg 67081, France.; CNRS UMR7242, Biotechnology and Cell Signaling, 300 bd Sébastien Brant, Illkirch 67412, France., Weber M; University of Strasbourg, 4 rue Blaise Pascal, Strasbourg 67081, France.; CNRS UMR7242, Biotechnology and Cell Signaling, 300 bd Sébastien Brant, Illkirch 67412, France., Velasco G; UMR7216 Epigénétique et Destin Cellulaire, CNRS, Université de Paris Cité, Epigenetics and Cell Fate, Lamarck building, 35 rue Hélène Brion, Paris F-75013, France., Francastel C; UMR7216 Epigénétique et Destin Cellulaire, CNRS, Université de Paris Cité, Epigenetics and Cell Fate, Lamarck building, 35 rue Hélène Brion, Paris F-75013, France.
Jazyk:	angličtina
Zdroj:	Human molecular genetics [Hum Mol Genet] 2024 Nov 20. Date of Electronic Publication: 2024 Nov 20.
DOI:	10.1093/hmg/ddae163
Abstrakt:	Since its discovery as a causative gene of the Immunodeficiency with Centromeric instability and Facial anomalies syndrome, ZBTB24 has emerged as a key player in DNA methylation, immunity and development. By extensively analyzing ZBTB24 genomic functions in ICF-relevant mouse and human cellular models, we document here its multiple facets as a transcription factor, with key roles in immune response-related genes expression and also in early embryonic development. Using a constitutive Zbtb24 ICF-like mutant and an auxin-inducible degron system in mouse embryonic stem cells, we showed that ZBTB24 is recruited to centromeric satellite DNA where it is required to establish and maintain the correct DNA methylation patterns through the recruitment of DNMT3B. The ability of ZBTB24 to occupy centromeric satellite DNA is conserved in human cells. Together, our results unveiled an essential and underappreciated role for ZBTB24 at mouse and human centromeric satellite repeat arrays by controlling their DNA methylation and transcription status. (© The Author(s) 2024. Published by Oxford University Press.)
Databáze:	MEDLINE
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