Comparative analysis of polysaccharide and cell wall structure in Aspergillus nidulans and Aspergillus fumigatus by solid-state NMR.

Autor: Gautam I; Department of Chemistry, Michigan State University, East Lansing, MI, USA., Yarava JR; Department of Chemistry, Michigan State University, East Lansing, MI, USA., Xu Y; Department of Chemistry, Michigan State University, East Lansing, MI, USA., Li R; Department of Chemistry, Michigan State University, East Lansing, MI, USA., Scott FJ; National High Magnetic Field Laboratory, Florida State University, Tallahassee, FL, USA., Mentink-Vigier F; National High Magnetic Field Laboratory, Florida State University, Tallahassee, FL, USA., Momany M; Fungal Biology Group & Department of Plant Biology, University of Georgia, Athens, GA, USA., Latgé JP; Institute of Molecular Biology and Biotechnology, University of Crete, Heraklion, Greece; Fungal Respiratory Infections Research Unit and SFR ICAT, University of Angers, France., Wang T; Department of Chemistry, Michigan State University, East Lansing, MI, USA. Electronic address: wangtuo1@msu.edu.
Jazyk: angličtina
Zdroj: Carbohydrate polymers [Carbohydr Polym] 2025 Jan 15; Vol. 348 (Pt A), pp. 122907. Date of Electronic Publication: 2024 Oct 26.
DOI: 10.1016/j.carbpol.2024.122907
Abstrakt: Invasive aspergillosis poses a significant threat to immunocompromised patients, leading to high mortality rates associated with these infections. Targeting the biosynthesis of cell wall carbohydrates is a promising strategy for antifungal drug development and will be advanced by a molecular-level understanding of the native structures of polysaccharides within their cellular context. Solid-state NMR spectroscopy has recently provided detailed insights into the cell wall organization of Aspergillus fumigatus, but genetic and biochemical evidence highlights species-specific differences among Aspergillus species. In this study, we employed a combination of 13 C, 15 N, and 1 H-detection solid-state NMR, supplemented by Dynamic Nuclear Polarization (DNP), to compare the structural organization of cell wall polymers and their assembly in the cell walls of A. fumigatus and A. nidulans, both of which are key model organisms and human pathogens. The two species exhibited a similar rigid core architecture, consisting of chitin, α-glucan, and β-glucan, which contributed to comparable cell wall properties, including polymer dynamics, water retention, and supramolecular organization. However, differences were observed in the chitin, galactosaminogalactan, protein, and lipid content, as well as in the dynamics of galactomannan and the structure of the glucan matrix.
Competing Interests: Declaration of competing interest The authors declare no conflict of interest.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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