High mechanical performance and multifunctional degraded fucoidan-derived bioink for 3D bioprinting.

Autor: Zhu S; College of Material Science and Engineering, Hunan University, Changsha 410082, China., Zhou Z; College of Biology, Hunan University, Changsha 410082, China. Electronic address: zhouzheng@hnu.edu.cn., Chen X; College of Material Science and Engineering, Hunan University, Changsha 410082, China., Zhu W; College of Material Science and Engineering, Hunan University, Changsha 410082, China., Yang M; College of Material Science and Engineering, Hunan University, Changsha 410082, China., Yu M; College of Material Science and Engineering, Hunan University, Changsha 410082, China., Sun J; College of Biology, Hunan University, Changsha 410082, China., Zuo Y; College of Biology, Hunan University, Changsha 410082, China., He J; College of Biology, Hunan University, Changsha 410082, China., Pan H; Shenzhen Key Laboratory of Marine Biomaterials, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China., Liu H; College of Material Science and Engineering, Hunan University, Changsha 410082, China. Electronic address: liuhairong@hnu.edu.cn.
Jazyk: angličtina
Zdroj: Carbohydrate polymers [Carbohydr Polym] 2025 Jan 15; Vol. 348 (Pt A), pp. 122805. Date of Electronic Publication: 2024 Sep 25.
DOI: 10.1016/j.carbpol.2024.122805
Abstrakt: While 3D bioprinting serves as a powerful tool in the field of tissue engineering, there is still a lack of natural biomaterial inks that simultaneously combine high mechanical performance with multiple biofunctionalities. Here, a single-component natural bioink with high strength and multi-biofunctionality was developed through the simple degradation and methacrylation of natural fucoidan. Hydrothermal degradation significantly decreased the natural fucoidan solution's viscosity by 99.9 %, meeting the necessary viscosity for Digital Light Processing (DLP) 3D printing. Meanwhile, various biofunctionalities of low molecular weight fucoidan obtained through degradation, such as antimicrobial and antioxidant properties, were developed. The resulting bioink exhibited good mechanical performance (compression modulus of 311 kPa), antimicrobial properties (antibacterial rates of 95.5 % and 97.9 % against E. coli and S. aureus, respectively), and antioxidant properties (intracellular ROS inhibition rates of 94.7 %). Using DLP 3D bioprinting, all printed products showed high shape fidelity with exceptional viability and activity of the encapsulated cells. Due to the unique sulfate structure resembling the natural components of chondroitin sulfate, the in vivo tests revealed its efficacy in promoting cartilage defect repair. In conclusion, the novel bioink blending high mechanical performance with multiple biofunctionalities, shows great potential in the 3D printing of tissue and organ regeneration.
Competing Interests: Declaration of competing interest The authors declare no competing financial interest.
(Copyright © 2024. Published by Elsevier Ltd.)
Databáze: MEDLINE
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