Effectiveness of the injectable hyaluronic acid-based microparticles loaded with cannabidiol on rat sciatic nerve injury model.

Autor: Davachi SM; Department of Biology and Chemistry, Texas A&M International University, Laredo, TX 78041, USA., Vazquez M; Department of Biology and Chemistry, Texas A&M International University, Laredo, TX 78041, USA., Soleimani M; Department of Medical Basic Sciences, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran., Hajmohammadi Z; Department of Tissue Engineering and Applied Cell Science, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Mohajer M; Eye Research Center, Five Senses Health Research Institute, School of Medicine, Iran University of Medical Sciences, Tehran, Iran; Faculty of Tissue Engineering, Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran., Jameie SB; Neuroscience Research Center (NRC), Iran University of Medical Sciences, Tehran, Iran; Department of Anatomical Sciences, School of Medicine, Iran University of Medical Sciences, Tehran, Iran., Khanmohammadi M; Skull Base Research Center, The Five Senses Institute, Hazrat Rasoul Akram Hospital, School of Medicine, Iran University of Medical Sciences, Tehran, Iran., Najafi R; Department of Life Science Engineering, Faculty of New Science and Technologies, University of Tehran, Iran., Bagher Z; ENT and Head and Neck Research Center and Department, The Five Senses Health Institute, School of Medicine, Iran University of Medical Sciences, Tehran, Iran; Department of Tissue Engineering and Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran., Hassanzadeh S; Eye Research Center, Five Senses Health Research Institute, School of Medicine, Iran University of Medical Sciences, Tehran, Iran; Neuroscience Research Center (NRC), Iran University of Medical Sciences, Tehran, Iran. Electronic address: sajadhassanzade528@gmail.com.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Nov 16; Vol. 283 (Pt 3), pp. 137780. Date of Electronic Publication: 2024 Nov 16.
DOI: 10.1016/j.ijbiomac.2024.137780
Abstrakt: We have developed an innovative peripheral nerve tissue repair approach by designing biomimetic microparticles loaded with cannabidiol (CBD) using horseradish peroxidase-mediated crosslinking within a microfluidic device. This method utilizes a water-in-oil emulsion system where a mixture of phenol-substituted hyaluronic acid (HAPh), CBD, and laccase is channeled into oil flow, forming hydrogel microparticles. The physical properties, such as their swelling rate, mechanical strength, and the sustained release of CBD, emphasize their potential in tissue engineering and drug delivery applications. Cellular proliferation studies within the microparticles demonstrate their cytocompatibility, making them suitable for developing microtissues. The microparticles also served as a controlled release mechanism for CBD-targeted delivery to the injured locations, showcasing the effectiveness and ability to aid in the regeneration of the sciatic nerve tissue. In vivo, histopathological analysis of treated sciatic nerve injuries showed enhanced axonal restoring and remyelination with HAPh microparticles containing CBD in contrast to control groups. Furthermore, microparticles enhanced various functional aspects of locomotor activities, such as functional sciatic index (SFI) values, response to heat stimulation, and muscle mass retention. In conclusion, results indicate that these composite biomimetic microparticles with CBD effectively promote nerve structural restoration and increase the reconstruction process in a sciatic nerve injury model.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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