HIV-1 budding requires cortical actin disassembly by the oxidoreductase MICAL1.

Autor:	Serrano T; Membrane Traffic and Cell Division Unit, Institut Pasteur, Université Paris Cité, CNRS UMR3691, Paris F-75015, France., Casartelli N; Virology department, Virus and Immunity Lab, Institut Pasteur, Université Paris Cité, Paris F-75015, France., Ghasemi F; Université Paris Cité, CNRS, Institut Jacques Monod, Paris F-75013, France., Wioland H; Université Paris Cité, CNRS, Institut Jacques Monod, Paris F-75013, France., Cuvelier F; Membrane Traffic and Cell Division Unit, Institut Pasteur, Université Paris Cité, CNRS UMR3691, Paris F-75015, France., Salles A; Institut Pasteur, Université Paris Cité, Photonic Bio-Imaging Unit, Centre de Ressources et Recherches Technologiques (UTechS-PBI, C2RT), Paris F-75015, France., Moya-Nilges M; Institut Pasteur, Université Paris Cité, Ultrastructural BioImaging, Paris F-75015, France., Welker L; Université de Strasbourg, Institut de Biologie Moléculaire et Cellulaire, Architecture et Réactivité de l'ARN, CNRS UPR9002, Strasbourg F-67084, France.; Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Department of Integrated Structural Biology, CNRS UMR 7104, Inserm U 1258, University of Strasbourg, Illkirch F-67404, France., Bernacchi S; Université de Strasbourg, Institut de Biologie Moléculaire et Cellulaire, Architecture et Réactivité de l'ARN, CNRS UPR9002, Strasbourg F-67084, France., Ruff M; Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Department of Integrated Structural Biology, CNRS UMR 7104, Inserm U 1258, University of Strasbourg, Illkirch F-67404, France., Jégou A; Université Paris Cité, CNRS, Institut Jacques Monod, Paris F-75013, France., Romet-Lemonne G; Université Paris Cité, CNRS, Institut Jacques Monod, Paris F-75013, France., Schwartz O; Virology department, Virus and Immunity Lab, Institut Pasteur, Université Paris Cité, Paris F-75015, France., Frémont S; Membrane Traffic and Cell Division Unit, Institut Pasteur, Université Paris Cité, CNRS UMR3691, Paris F-75015, France., Echard A; Membrane Traffic and Cell Division Unit, Institut Pasteur, Université Paris Cité, CNRS UMR3691, Paris F-75015, France.
Jazyk:	angličtina
Zdroj:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2024 Nov 26; Vol. 121 (48), pp. e2407835121. Date of Electronic Publication: 2024 Nov 18.
DOI:	10.1073/pnas.2407835121
Abstrakt:	Many enveloped viruses bud from the plasma membrane that is tightly associated with a dense and thick actin cortex. This actin network represents a significant challenge for membrane deformation and scission, and how it is remodeled during the late steps of the viral cycle is largely unknown. Using superresolution microscopy, we show that HIV-1 buds in areas of the plasma membrane with low cortical F-actin levels. We find that the cellular oxidoreductase MICAL1 locally depolymerizes actin at budding sites to promote HIV-1 budding and release. Upon MICAL1 depletion, F-actin abnormally remains at viral budding sites, incompletely budded viruses accumulate at the plasma membrane and viral release is impaired. Remarkably, normal viral release can be restored in MICAL1-depleted cells by inhibiting Arp2/3-dependent branched actin networks. Mechanistically, we find that MICAL1 directly disassembles branched-actin networks and controls the timely recruitment of the Endosomal Sorting Complexes Required for Transport scission machinery during viral budding. In addition, the MICAL1 activator Rab35 is recruited at budding sites, functions in the same pathway as MICAL1, and is also required for viral release. This work reveals a role for oxidoreduction in triggering local actin depolymerization to control HIV-1 budding, a mechanism that may be widely used by other viruses. The debranching activity of MICAL1 could be involved beyond viral budding in various other cellular functions requiring local plasma membrane deformation. Competing Interests: Competing interests statement:The authors declare no competing interest.
Databáze:	MEDLINE
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