Microbiota composition of Culex perexiguus mosquitoes during the West Nile virus outbreak in southern Spain.

Autor: Garrigós M; Department of Conservation Biology and Global Change, Estación Biológica de Doñana (EBD), CSIC, Sevilla, Spain., Garrido M; Department of Parasitology, Faculty of Pharmacy, University of Granada, Granada, Spain., Ruiz-López MJ; Department of Conservation Biology and Global Change, Estación Biológica de Doñana (EBD), CSIC, Sevilla, Spain.; CIBER de Epidemiología y Salud Pública (CIBERESP), Madrid, Spain., García-López MJ; Department of Endocrinology and Nutrition, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Universitario Virgen de la Victoria, Málaga, Spain.; Facultad de Medicina, Universidad de Málaga, Málaga, Spain., Veiga J; Department of Conservation Biology and Global Change, Estación Biológica de Doñana (EBD), CSIC, Sevilla, Spain., Magallanes S; Department of Conservation Biology and Global Change, Estación Biológica de Doñana (EBD), CSIC, Sevilla, Spain.; CIBER de Epidemiología y Salud Pública (CIBERESP), Madrid, Spain., Soriguer R; Department of Conservation Biology and Global Change, Estación Biológica de Doñana (EBD), CSIC, Sevilla, Spain.; CIBER de Epidemiología y Salud Pública (CIBERESP), Madrid, Spain., Moreno-Indias I; Department of Endocrinology and Nutrition, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Universitario Virgen de la Victoria, Málaga, Spain.; CIBER de Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Madrid, Spain., Figuerola J; Department of Conservation Biology and Global Change, Estación Biológica de Doñana (EBD), CSIC, Sevilla, Spain.; CIBER de Epidemiología y Salud Pública (CIBERESP), Madrid, Spain., Martínez-de la Puente J; Department of Conservation Biology and Global Change, Estación Biológica de Doñana (EBD), CSIC, Sevilla, Spain.; CIBER de Epidemiología y Salud Pública (CIBERESP), Madrid, Spain.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2024 Nov 18; Vol. 19 (11), pp. e0314001. Date of Electronic Publication: 2024 Nov 18 (Print Publication: 2024).
DOI: 10.1371/journal.pone.0314001
Abstrakt: West Nile virus (WNV) is a flavivirus naturally circulating between mosquito vectors and birds, occasionally infecting horses and humans and causing epidemiologically relevant outbreaks. In Spain, the first big WNV outbreak was recorded in 2020, resulting in 77 people infected and 8 fatalities, most of them in southern Spain. Culex perexiguus was identified as the primary vector of WNV maintaining its enzootic circulation of the virus. Growing evidence highlights the role of mosquito microbiota as a key component determining the vectorial capacity of mosquitoes, largely contributing to disease epidemiology. Here, we develop, to our knowledge, the first identification of the microbiota composition of this mosquito vector under natural conditions and test for the potential relationship between mosquito microbiota composition and WNV infection. To do so, we collected mosquitoes in a natural area of southern Spain during the 2020 WNV outbreak and identified the microbiota composition of mosquitoes using a 16S rRNA gene metabarcoding approach. The microbiota of Cx. perexiguus was dominated by the phylum Proteobacteria. The most abundant families were Burkholderiaceae and Erwiniaceae, including the genera Burkholderia, Erwinia, and Pantoea. The genus Wolbachia, which use to dominate the microbiota of Cx. pipiens and negatively interact with WNV according to the literature, had a low prevalence and relative abundance in Cx. perexiguus and its abundance did not differ between WNV-positive and WNV-negative mosquito pools. The microbiota diversity and composition of Cx. perexiguus were not significantly related to the WNV infection status. These results provide the first identification of the mosquito microbiota in an endemic area of WNV circulation in Spain.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Garrigós et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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