Collagen binding adhesin restricts Staphylococcus aureus skin infection.

Autor: Bhattacharya M; Department of Immunology and Microbiology, University of Colorado School of Medicine, Aurora CO, USA., Spencer BL; Department of Immunology and Microbiology, University of Colorado School of Medicine, Aurora CO, USA., Kwiecinski JM; Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland., Podkowik M; Department of Medicine, Division of Infectious Diseases and Immunology, New York University Grossman School of Medicine, New York, NY, USA.; Antimicrobial-Resistant Pathogens Program, New York University Grossman School of Medicine, New York, NY, USA., Putzel G; Department of Microbiology, New York University Grossman School of Medicine, New York, NY, USA.; Antimicrobial-Resistant Pathogens Program, New York University Grossman School of Medicine, New York, NY, USA., Pironti A; Department of Microbiology, New York University Grossman School of Medicine, New York, NY, USA.; Antimicrobial-Resistant Pathogens Program, New York University Grossman School of Medicine, New York, NY, USA., Shopsin B; Department of Microbiology, New York University Grossman School of Medicine, New York, NY, USA.; Department of Medicine, Division of Infectious Diseases and Immunology, New York University Grossman School of Medicine, New York, NY, USA.; Antimicrobial-Resistant Pathogens Program, New York University Grossman School of Medicine, New York, NY, USA., Doran KS; Department of Immunology and Microbiology, University of Colorado School of Medicine, Aurora CO, USA., Horswill AR; Department of Immunology and Microbiology, University of Colorado School of Medicine, Aurora CO, USA.; Department of Veterans Affairs, Eastern Colorado Healthcare System, Denver, CO, USA.; Lead author.
Jazyk: angličtina
Zdroj: BioRxiv : the preprint server for biology [bioRxiv] 2024 Nov 02. Date of Electronic Publication: 2024 Nov 02.
DOI: 10.1101/2024.11.01.621145
Abstrakt: Staphylococcus aureus causes approximately 80% of skin and soft tissue infections (SSTIs). Collagen is the most abundant human extracellular matrix protein with critical roles in wound healing, and S. aureus encodes a collagen binding adhesin (Cna). The role of this protein during skin infections is unknown. Here we report that inability to bind collagen results in worsened pathology of intradermal Δ cna S. aureus infection. WT/Cna+ S. aureus showed reduced infection severity, aggregate formation, and significantly improved clearance of bacteria. Cna binds to the collagen-like domain of serum C1q protein to reduce its opsonophagocytic functions. We demonstrate that infection of C1qKO mice with WT bacteria show results similar to the Δ cna group. Conversely, inability to bind collagen resulted in an amplified inflammatory response caused in part by macrophage and neutrophil small molecule mediators released at the infection site (MMP-9, MMP-12, LTB 4 ), resulting in increased immune cell infiltration and death.
Competing Interests: Declaration of interests. The authors declare no competing interests.
Databáze: MEDLINE
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