Structural characterization of an arabinogalactan rich fraction from Bauhinia forficata link leaves and evaluation of its effect on THP-1 macrophages.

Autor: Campo-Grande GC; Biochemistry and Molecular Biology Department, Federal University of Paraná, CEP 81531-980 Curitiba, PR, Brazil., D'Agostin JB; Laboratory of Inflammatory and Neoplasic Cells, Cell Biology Department, Federal University of Paraná, CEP 81531-980 Curitiba, PR, Brazil., de Santana Filho AP; Biochemistry and Molecular Biology Department, Federal University of Paraná, CEP 81531-980 Curitiba, PR, Brazil., da Silva Mello G; Biochemistry and Molecular Biology Department, Federal University of Paraná, CEP 81531-980 Curitiba, PR, Brazil., Benedetti PR; Biochemistry and Molecular Biology Department, Federal University of Paraná, CEP 81531-980 Curitiba, PR, Brazil., Dos Santos WF; Biochemistry and Molecular Biology Department, Federal University of Paraná, CEP 81531-980 Curitiba, PR, Brazil., Simas FF; Laboratory of Inflammatory and Neoplasic Cells, Cell Biology Department, Federal University of Paraná, CEP 81531-980 Curitiba, PR, Brazil., Cipriani TR; Biochemistry and Molecular Biology Department, Federal University of Paraná, CEP 81531-980 Curitiba, PR, Brazil. Electronic address: trcipriani@ufpr.br.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Nov 15, pp. 137731. Date of Electronic Publication: 2024 Nov 15.
DOI: 10.1016/j.ijbiomac.2024.137731
Abstrakt: Bauhinia forficata is a medicinal plant known as cow's paw, used for many purposes. Although there are studies that aimed to elucidate compounds from the plant leaves, there is no information about its polysaccharides. This study intended to obtain a polysaccharide rich fraction from its leaves, structurally characterize the water-soluble polysaccharides, as well as evaluate their effect on THP-1 cells. From the aqueous extract, followed by purification processes, a polysaccharide fraction (TCAS) was obtained, constituted mainly of arabinose and galactose. Bidimensional NMR ( 13 C/ 1 H, HSQC) and methylation analyses identified type I and type II arabinogalactans, arabinan and starch as the major polysaccharides of the fraction. TCA-S was then submitted to starch removal process and renamed as TCASα. TCASα (2 to 500 μg/mL) was not cytotoxic to THP-1-cells and exhibited an immunostimulatory effect by increasing the secretion of nitric oxide and both pro-inflammatory cytokine IL-1β and anti-inflammatory cytokine IL-10. Immunomodulatory effect on IL-6 secretion was observed when macrophages were treated with TCA-Sα at 500 μg/mL. Additionally, the ratio between the concentrations of pro and anti-inflammatory cytokines produced by LPS-treated cells was higher than that produced by LPS plus TCA-Sα treated ones, suggesting that the polysaccharide fraction could modulate the LPS inflammation effects.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier B.V.)
Databáze: MEDLINE
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