Characterization of heat- and alkali-resistant feruloyl esterase from Humicola insolens and application in the production of high-strength kraft straws.

Autor: Wang QY; School of Bioengineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, Shandong, PR China; State Key Laboratory of Bio-based Materials and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, Shandong, PR China., Liu XJ; School of Bioengineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, Shandong, PR China; State Key Laboratory of Bio-based Materials and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, Shandong, PR China., Hou YH; School of Bioengineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, Shandong, PR China; State Key Laboratory of Bio-based Materials and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, Shandong, PR China. Electronic address: houyunhua@qlu.edu.cn., Wang JZ; School of Bioengineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, Shandong, PR China., Lin MZ; School of Bioengineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, Shandong, PR China; State Key Laboratory of Bio-based Materials and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, Shandong, PR China., Yang QZ; School of Bioengineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, Shandong, PR China; State Key Laboratory of Bio-based Materials and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, Shandong, PR China. Electronic address: yqz@qlu.edu.cn.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Dec; Vol. 283 (Pt 2), pp. 137742. Date of Electronic Publication: 2024 Nov 17.
DOI: 10.1016/j.ijbiomac.2024.137742
Abstrakt: The application of feruloyl esterase (FAE) in biobleaching can effectively hydrolyze the feruloyl ester bond between hemicellulose and lignin, thereby partially disrupting the dense structure of the fiber, reducing the use of chemical reagents, and obtaining high-performance pulp fibers. Here, we successfully expressed the thermostable alkaline feruloyl esterase from Humicola insolens (H. insolens) in Pichia pastoris GS115, with an enzyme activity yield of 2.36 ± 0.21 U/mL. The highest activity of FAE for the hydrolysis of ethyl ferulate was observed at pH 7.5 and 70 °C. It retained about 56 % of its maximum activity in the pH range of 11.0. After being incubated at 50-55 °C for 1 h, it retained 70.05 % of its maximum activity. The addition of bio-enzyme pretreatment before chemical bleaching can reduce chemical reagents by 20 %, result in a 10.64 % reduction in kappa value, and increase the delignification rate of the pulp by 6.36 %. In addition, the surface of the enzyme-treated pulp showed rougher broom-like fiber filaments, and the viscosity of the enzyme-pretreated pulp (ECP) before chemical treatment increased by 131.51 % compared to the chemically treated pulp (CP), further indicating that the enzyme-pretreated pulp had higher pulp strength.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier B.V.)
Databáze: MEDLINE
Externí odkaz: