MCM10: A potential biomarker for cervical cancer and precancerous lesions.

Autor: Ahmed SM; Cell Biology and Molecular Genetics, Yenepoya Research Centre, Yenepoya (Deemed to be University), Mangalore 575018, India. Electronic address: sumayyahmqa12@gmail.com., Laha S; Cell Biology and Molecular Genetics, Yenepoya Research Centre, Yenepoya (Deemed to be University), Mangalore 575018, India. Electronic address: lahasuparna@gmail.com., Ifthikar MA; Zulekha Yenepoya Institute of Oncology, Yenepoya (Deemed to be University), Mangalore 575018, India. Electronic address: anjumifthikar@yenepoya.edu.in., Das R; Data Analytics, Bioinformatics and Structural Biology, Yenepoya Research Centre, Yenepoya (Deemed to be University), Mangalore, 575018, India. Electronic address: das.ranajit@gmail.com., Das SP; Cell Biology and Molecular Genetics, Yenepoya Research Centre, Yenepoya (Deemed to be University), Mangalore 575018, India. Electronic address: shandas76@gmail.com.
Jazyk: angličtina
Zdroj: Gene [Gene] 2025 Feb 05; Vol. 936, pp. 149103. Date of Electronic Publication: 2024 Nov 17.
DOI: 10.1016/j.gene.2024.149103
Abstrakt: Cervical cancer remains a significant health burden worldwide, emphasizing the need for early detection and intervention. DNA replication is perturbed in cancer cells, and the minichromosome maintenance protein 10 plays an important role in origin firing. By analyzing the MCM10 mRNA expression in healthy controls, precancerous lesions, and cervical cancer using qRT-PCR, we can infer if it can be considered a biomarker. We collected cervical smear samples from patients and performed MCM10 expression analysis to set up thresholds for risk stratification. We also investigated the HPV status among the patient samples with precancerous lesions and cervical cancer and found 70 % of them to be positive. Our results demonstrated a significant upregulation of MCM10 mRNA expression in tumor samples (n = 40, 7.83 ± 1.2) and precancerous lesions (n = 54, 5.69 ± 1.4) compared to normal (n = 50, 4.27 ± 0.80) with a R 2 value of 0.59, confirming its role in the progression and development of cervical cancer. In conclusion, this study emphasizes the potential role of MCM10 as a biomarker. Our study would improve early detection rates, and we propose MCM10-based community screening for risk stratification, prevention, and prognosis.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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