Upregulation of inhibitor of DNA binding 1 and 3 is important for efficient thermogenic response in human adipocytes.

Autor: Arianti R; Laboratory of Cell Biochemistry, Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, Debrecen, 4032, Hungary.; Universitas Muhammadiyah Bangka Belitung, Pangkalpinang, 33134, Indonesia., Vinnai BÁ; Laboratory of Cell Biochemistry, Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, Debrecen, 4032, Hungary.; Doctoral School of Molecular Cell and Immune Biology, University of Debrecen, Debrecen, 4032, Hungary., Alrifai R; Laboratory of Cell Biochemistry, Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, Debrecen, 4032, Hungary.; Doctoral School of Molecular Cell and Immune Biology, University of Debrecen, Debrecen, 4032, Hungary., Karadsheh G; Laboratory of Cell Biochemistry, Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, Debrecen, 4032, Hungary.; Doctoral School of Molecular Cell and Immune Biology, University of Debrecen, Debrecen, 4032, Hungary., Al-Khafaji YQ; Laboratory of Cell Biochemistry, Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, Debrecen, 4032, Hungary., Póliska S; Genomic Medicine and Bioinformatics Core Facility, Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, Debrecen, 4032, Hungary., Győry F; Department of Surgery, Faculty of Medicine, University of Debrecen, Debrecen, 4032, Hungary., Fésüs L; Laboratory of Cell Biochemistry, Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, Debrecen, 4032, Hungary. fesus@med.unideb.hu., Kristóf E; Laboratory of Cell Biochemistry, Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, Debrecen, 4032, Hungary. kristof.endre@med.unideb.hu.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Nov 16; Vol. 14 (1), pp. 28272. Date of Electronic Publication: 2024 Nov 16.
DOI: 10.1038/s41598-024-79634-2
Abstrakt: Brown and beige adipocytes can be activated by β-adrenergic agonist via cAMP-dependent signaling. Performing RNA-sequencing analysis in human cervical area-derived adipocytes, we found that dibutyryl-cAMP, which can mimic in vivo stimulation of browning and thermogenesis, enhanced the expression of browning and batokine genes and upregulated several signaling pathway genes linked to thermogenesis. We observed that the expression of inhibitor of DNA binding and cell differentiation (ID) 1 and particularly ID3 was strongly induced by the adrenergic stimulation. The degradation of ID1 and ID3 elicited by the ID antagonist AGX51 during thermogenic activation prevented the induction of proton leak respiration that reflects thermogenesis and abrogated cAMP analogue-stimulated upregulation of thermogenic genes and mitochondrial complex I, II, and IV subunits, independently of the proximal cAMP-PKA signaling pathway. The presented data suggests that ID proteins contribute to efficient thermogenic response of adipocytes during adrenergic stimulation.
Competing Interests: Declarations Competing interests The authors declare no competing interests.
(© 2024. The Author(s).)
Databáze: MEDLINE
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