SITP: A single cell bioinformatics analysis flow captures proteasome markers in the development of breast cancer.

Autor: Zhou XJ; the First Department of Breast Cancer, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin 300060, PR China; Key Laboratory of Breast Cancer Prevention and Therapy, Tianjin Medical University, Ministry of Education, Tianjin 300060, PR China; Key Laboratory of Cancer Prevention and Therapy, Tianjin 300060, PR China; Tianjin's Clinical Research Center for Cancer, Tianjin 300060, PR China., Liu XF; the First Department of Breast Cancer, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin 300060, PR China; Key Laboratory of Breast Cancer Prevention and Therapy, Tianjin Medical University, Ministry of Education, Tianjin 300060, PR China; Key Laboratory of Cancer Prevention and Therapy, Tianjin 300060, PR China; Tianjin's Clinical Research Center for Cancer, Tianjin 300060, PR China., Wang X; the First Department of Breast Cancer, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin 300060, PR China; Key Laboratory of Breast Cancer Prevention and Therapy, Tianjin Medical University, Ministry of Education, Tianjin 300060, PR China; Key Laboratory of Cancer Prevention and Therapy, Tianjin 300060, PR China; Tianjin's Clinical Research Center for Cancer, Tianjin 300060, PR China. Electronic address: wangxin@tjmuch.com., Cao XC; the First Department of Breast Cancer, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin 300060, PR China; Key Laboratory of Breast Cancer Prevention and Therapy, Tianjin Medical University, Ministry of Education, Tianjin 300060, PR China; Key Laboratory of Cancer Prevention and Therapy, Tianjin 300060, PR China; Tianjin's Clinical Research Center for Cancer, Tianjin 300060, PR China. Electronic address: caoxuchen@tmu.edu.cn.
Jazyk: angličtina
Zdroj: Methods (San Diego, Calif.) [Methods] 2025 Jan; Vol. 233, pp. 1-10. Date of Electronic Publication: 2024 Nov 15.
DOI: 10.1016/j.ymeth.2024.11.011
Abstrakt: Single cell sequencing and related databases have been widely used in the exploration of cancer occurrence and development, but there is still no in-depth explanation of specific and complicated cellular protein modification processes. Ubiquitin-Proteasome System (UPS), as a specific and precise protein modification and degradation process, plays an important role in the biological functions of cancer cell proliferation and apoptosis. Proteasomes, vital multi-catalytic proteinases in eukaryotic cells, play a crucial role in protein degradation and contribute to tumor regulation. The 26S proteasome, part of the ubiquitin-proteasome system. In this study, we have enrolled a common SITP process including analysis of single cell sequencing to elucidate a flow that can capture typical proteasome markers in the oncogenesis and progression of breast cancer. PSMD11, a key component of the 26S proteasome regulatory particle, has been identified as a critical survival factor in cancer cells. Results suggest that PSMD11's rapid degradation is linked to acute apoptosis in cancer cells, making it a potential target for cancer treatment. Our study explored the potential mechanisms of PSMD11 in breast cancer development. The findings revealed the feasibility of disclosing ubiquitinating biomarkers from public database, as well as presented new evidence supporting PSMD11 as a potential therapeutic biomarker for breast cancer.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier Inc.)
Databáze: MEDLINE
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