Enduring effects of acute prenatal ischemia in rat soleus muscle, and protective role of erythropoietin.

Autor: Sancerni T; Univ. Lille, Univ Artois, Univ Littoral Côte d'Opale, URePSSS - Unité de Recherche Pluridisciplinaire Sport Santé Société, ULR7369, Lille, F-59000, France., Montel V; Univ. Lille, Univ Artois, Univ Littoral Côte d'Opale, URePSSS - Unité de Recherche Pluridisciplinaire Sport Santé Société, ULR7369, Lille, F-59000, France., Dereumetz J; Univ. Lille, Univ Artois, Univ Littoral Côte d'Opale, URePSSS - Unité de Recherche Pluridisciplinaire Sport Santé Société, ULR7369, Lille, F-59000, France., Cochon L; Univ. Lille, Univ Artois, Univ Littoral Côte d'Opale, URePSSS - Unité de Recherche Pluridisciplinaire Sport Santé Société, ULR7369, Lille, F-59000, France., Coq JO; Institut des Sciences du Mouvement (ISM), Team 'Plasticité des Systèmes Nerveux et Musculaires', UMR 7287 CNRS, Aix-Marseille Université Faculté des Sports, Marseille Cedex 09, F-13288, France., Bastide B; Univ. Lille, Univ Artois, Univ Littoral Côte d'Opale, URePSSS - Unité de Recherche Pluridisciplinaire Sport Santé Société, ULR7369, Lille, F-59000, France., Canu MH; Univ. Lille, Univ Artois, Univ Littoral Côte d'Opale, URePSSS - Unité de Recherche Pluridisciplinaire Sport Santé Société, ULR7369, Lille, F-59000, France. marie-helene.canu@univ-lille.fr.
Jazyk: angličtina
Zdroj: Journal of muscle research and cell motility [J Muscle Res Cell Motil] 2024 Nov 16. Date of Electronic Publication: 2024 Nov 16.
DOI: 10.1007/s10974-024-09684-6
Abstrakt: Motor disorders are considered to originate mainly from brain lesions. Placental dysfunction or maternal exposure to a persistently hypoxic environment is a major cause of further motor disorders such as cerebral palsy. Our main goal was to determine the long-term effects of mild intrauterine acute ischemic stress on rat soleus myofibres and whether erythropoietin treatment could prevent these changes. Rat embryos were subjected to ischemic stress at embryonic day E17. They then received an intraperitoneal erythropoietin injection at postnatal days 1-5. Soleus muscles were collected at postnatal day 28. Prenatal ischemic stress durably affected muscle structure, as indicated by the greater fiber cross-sectional area (+ 18%) and the greater number of mature vessels (i.e. vessels with mature endothelial cells) per myofibres (+ 43%), and muscle biochemistry, as shown by changes in signaling pathways involved in protein synthesis/degradation balance (-81% for 4EBP1; -58% for AKT) and Hif1α expression levels (+ 95%). Erythropoietin injection in ischemic pups had a weak protective effect: it increased muscle mass (+ 25% with respect to ischemic pups) and partially prevented the increase in muscle degradation pathways and mature vascularization, whereas it exacerbated the decrease in synthesis pathways. Hence, erythropoietin treatment after acute ischemic stress contributes to muscle adaptation to ischemic conditions.
Competing Interests: Declarations Ethical approval We confirm that all procedures were carried out in accordance with the European Communities Council Directive 2010/63/UE, and were approved by the Regional Committee on the Ethics of Animal Experiments of the Nord Pas-de-Calais region (CEEA 75, reference number: APAFIS#4732-2016031 112395755v7). Competing interests The authors declare no competing interests.
(© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
Databáze: MEDLINE
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