Genetic diversity of Theileria spp. in deer (Artiodactyla: Cervidae) from Brazil.

Autor: Calchi AC; Vector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, School of Agricultural and Veterinarian Sciences (FCAV), São Paulo State University (UNESP), Campus de Jaboticabal, Via de Acesso Prof. Paulo Donato Castellane, S/N, Zona Rural, CEP, Jaboticabal, São Paulo, 14884-900, Brazil., Duarte JMB; Deer Research and Conservation Center (NUPECCE), São Paulo State University (UNESP), Jaboticabal, SP, Brazil., Castro-Santiago AC; Department of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine and Animal Science of University of São Paulo (FMVZ-USP), São Paulo, SP, Brazil., Bassini-Silva R; Laboratório de Coleções Zoológicas, Instituto Butantan, São Paulo, SP, Brazil., Barros-Battesti DM; Vector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, School of Agricultural and Veterinarian Sciences (FCAV), São Paulo State University (UNESP), Campus de Jaboticabal, Via de Acesso Prof. Paulo Donato Castellane, S/N, Zona Rural, CEP, Jaboticabal, São Paulo, 14884-900, Brazil., Machado RZ; Vector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, School of Agricultural and Veterinarian Sciences (FCAV), São Paulo State University (UNESP), Campus de Jaboticabal, Via de Acesso Prof. Paulo Donato Castellane, S/N, Zona Rural, CEP, Jaboticabal, São Paulo, 14884-900, Brazil., André MR; Vector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, School of Agricultural and Veterinarian Sciences (FCAV), São Paulo State University (UNESP), Campus de Jaboticabal, Via de Acesso Prof. Paulo Donato Castellane, S/N, Zona Rural, CEP, Jaboticabal, São Paulo, 14884-900, Brazil. mr.andre@unesp.br.
Jazyk: angličtina
Zdroj: Parasitology research [Parasitol Res] 2024 Nov 16; Vol. 123 (11), pp. 384. Date of Electronic Publication: 2024 Nov 16.
DOI: 10.1007/s00436-024-08398-5
Abstrakt: Babesia spp. and Theileria spp. are tick-borne apicomplexan protozoa that can cause disease in animals and humans. Deer are considered reservoirs for a wide variety of Piroplasmida species, including some potentially zoonotic. This study aimed to investigate the occurrence and genetic diversity of piroplasmids in wild deer sampled in four Brazilian states (São Paulo, Mato Grosso do Sul, Paraná and Goiás). For this purpose, extracted DNA samples from 181 deer buffy coat samples (138 Blastocerus dichotomus, 26 Subulo gouazoubira, 4 Mazama jucunda, 3 Mazama rufa and 10 Ozotocerus bezoarticus) were subjected to a nested PCR (nPCR) assay based on the 18S rRNA gene in order to perform a screening for piroplasmids and characterized based on the near-complete 18S rRNA, hsp70 and cox-3 genes. As a result, 75.14% (136/181) samples were positive for piroplasmids. Of these, 108 (79.41%), 101 (74.26%) and 67 (49.26%) were positive to near complete 18S rRNA, hsp70 and cox-3 genes, respectively. Phylogenetic analyses based on three molecular markers showed similar topology to each other. All sequences obtained in the present study were positioned into the Theileria sensu stricto clade, forming a distinct clade, albeit close to T. cervi. Most sequences grouped together into a large clade divided into subclades, which were often related to deer genus/species, showing that Theileria lineages seemed to show specificity according to deer genus/species. Two 18S rRNA sequences (one obtained from S. gouazoubira and another from M. jucunda) were positioned into a different clade, apart from other sequences detected in this study, indicating that different species of Theileria occur in deer from Brazil. Two subclusters were observed in the phylogenetic analysis based on the hsp70 gene: the first containing only sequences detected in marsh deer and the second grouping sequences detected in brocket deer (Mazama spp. and S. gouazoubira). The latter was also divided into smaller clades that grouped Theileria genotypes according to deer species (M. jucunda, M. rufa and S. gouazoubira). This study provides the first molecular evidence of Theileria infection in M. jucunda, as well as co-infection by distinct Theileria (sub)species/genotypes in the same deer was evidenced. Finally, this study expanded the knowledge on the diversity of Theileria spp. infecting deer from South America.
Competing Interests: Declarations Ethical approval All animal procedures and management protocols were approved by IBAMA (license number 10636–1) and the Ethics Committee on Animal Use of the School of Agricultural and Veterinarian Scie1nces (FCAV/UNESP) (protocol number 9412/2022). Consent to participate and consent for publication Not applicable. Conflict of interest The authors declare no competing interests.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
Externí odkaz: