Folic Acid Attenuates N-Methyl-N'-Nitro-N-NitrosoguanidineInduced Gastric Mucosal Injury in Rats.

Autor:	Peng C; North Sichuan Medical College, Nanchong, China., Wang L; North Sichuan Medical College, Nanchong, China., Liang Y; North Sichuan Medical College, Nanchong, China., Che L; North Sichuan Medical College, Nanchong, China., Sun R; North Sichuan Medical College, Nanchong, China., Yu J; North Sichuan Medical College, Nanchong, China., Gong J; North Sichuan Medical College, Nanchong, China., Wang D; North Sichuan Medical College, Nanchong, China., Cheng S; North Sichuan Medical College, Nanchong, China., Yang Q; North Sichuan Medical College, Nanchong, China., Jing T; Huazhong University of Science and Technology, Wuhan, Hubei, China., Liu Z; North Sichuan Medical College, Nanchong, China.
Jazyk:	angličtina
Zdroj:	The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology [Turk J Gastroenterol] 2024 Nov 01; Vol. 35 (11), pp. 839-848.
DOI:	10.5152/tjg.2024.23506
Abstrakt:	Background/aims: N-Methyl-N'-nitroso-N-nitrosoguanidine (MNNG) is suspected to increase the risk of developing stomach cancer. Folic acid (FA) is familiar with decreasing inflammation. We expected that FA would protect against MNNG-induced gastric mucosal injury. Materials and Methods: Thirty 12-week-old SPF-grade female Sprague-Dawley (SD) rats were treated with MNNG and given different dosages of FA as an intervention measure. Quantitative polymerase chain reaction (qPCR) was used to analyze the expression of IL-1, IL-6, IL-8, IL-18, TNF-α, NLRP3, ASC, and caspase-1 genes. The enzyme-linked immunosorbent assay (ELISA) was utilized for the identification of inflammatory cytokines. Western blot was accustomed to detecting IL-1β, IL-18, and NLRP3 inflammatory vesicles in gastric tissue. Furthermore, the gastric mucosal tissues underwent histological examination. Results: Our investigation demonstrated that FA reduced MNNG-induced inflammatory factor increase by decreasing NF-κB signaling (P < .05). Furthermore, FA prevented the MNNG-induced upregulation of NLRP3 inflammasome-related genes and proteins (all P < .01). Conclusion: Our data imply that MNNG exposure stimulates the NF-κB/NLRP3 pathway, while FA suppresses it, limiting stomach mucosal inflammation.
Databáze:	MEDLINE
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