Blood CD45 + /CD3 + lymphocyte-released extracellular vesicles and mortality in hospitalized patients with coronavirus disease 2019.

Autor: Suades R; Institut de Recerca Sant Pau (IR SANT PAU), Barcelona, Spain.; Centro de Investigación Biomédica en Red Enfermedades Cardiovasculares (CIBERCV), Institute of Health Carlos III (ISCIII), Madrid, Spain., Greco MF; Institut de Recerca Sant Pau (IR SANT PAU), Barcelona, Spain.; Department of Clinical Sciences and Community Health, Università Degli Studi di Milano, Milan, Italy., Padró T; Institut de Recerca Sant Pau (IR SANT PAU), Barcelona, Spain.; Centro de Investigación Biomédica en Red Enfermedades Cardiovasculares (CIBERCV), Institute of Health Carlos III (ISCIII), Madrid, Spain., de Santisteban V; Institut de Recerca Sant Pau (IR SANT PAU), Barcelona, Spain., Domingo P; Infectious Diseases Unit, Department of Internal Medicine, Hospital de la Santa Creu i Sant Pau-IR SANT PAU, Universitat Autònoma de Barcelona (UAB), Barcelona, Spain., Benincasa G; Institut de Recerca Sant Pau (IR SANT PAU), Barcelona, Spain.; Department of Advanced Medical and Surgical Sciences (DAMSS), University of Campania 'Luigi Vanvitelli', Naples, Italy., Napoli C; Department of Advanced Medical and Surgical Sciences (DAMSS), University of Campania 'Luigi Vanvitelli', Naples, Italy.; Department of Internal Medicine and Specialistics, Division of Clinical Immunology, Immunohematology, Transfusion Medicine and Transplant Immunology (SIMT), Azienda Universitaria Policlinico (AOU), Naples, Italy., Greco S; Molecular Cardiology Laboratory, IRCCS Policlinico San Donato, Milan, Italy., Madè A; Molecular Cardiology Laboratory, IRCCS Policlinico San Donato, Milan, Italy., Ranucci M; Department of Cardiovascular Anesthesia and Intensive Care, IRCCS Policlinico San Donato, Milan, Italy., Devaux Y; Cardiovascular Research Unit, Department of Precision Health, Luxembourg Institute of Health, Strassen, Luxembourg., Martelli F; Molecular Cardiology Laboratory, IRCCS Policlinico San Donato, Milan, Italy., Badimon L; Institut de Recerca Sant Pau (IR SANT PAU), Barcelona, Spain.; Centro de Investigación Biomédica en Red Enfermedades Cardiovasculares (CIBERCV), Institute of Health Carlos III (ISCIII), Madrid, Spain.; Cardiovascular Research Chair, UAB, Barcelona, Spain.
Jazyk: angličtina
Zdroj: European journal of clinical investigation [Eur J Clin Invest] 2024 Nov 15, pp. e14354. Date of Electronic Publication: 2024 Nov 15.
DOI: 10.1111/eci.14354
Abstrakt: Background: The global pandemic of coronavirus disease 2019 (COVID-19) represented a major public health concern. Growing evidence shows that plasma of COVID-19 patients contains large numbers of circulating extracellular vesicles (cEVs) that correlate with disease severity and recovery. In this study, we sought to characterize the longitudinal cEV signature in critically ill COVID-19 patients during hospitalization and its relation to mortality risk.
Methods: cEVs were quantitatively and phenotypically analysed in hospitalized non-surviving COVID-19 patients at baseline (n = 42) and before exitus (n = 40) and in 40 healthy volunteers as a reference group by high sensitivity nano flow cytometry using specific markers for parental cell sources and activation.
Results: Levels of cEV subtypes differed between patients with severe COVID-19 and healthy subjects, specifically those from platelets and endothelial, inflammatory and viral infected cells, which associate to high mortality risk. In the longitudinal analysis from baseline to the time point immediately preceding death, no changes were found for platelet, pan-leukocyte, and lung epithelial cell-shed cEVs, while endothelial cell releases of EVs (eEVs) significantly differed. Vascular endothelial growth factor receptor 2-positive eEVs were significantly increased before death compared to admission whereas endoglin and E-selectin-containing eEVs did not change. Conversely, lymphocyte (ℓEV), monocyte, macrophage, pericyte and progenitor cell-derived cEVs displayed significant reductions before exitus. Noteworthy, levels of CD45 + /CD3 + -ℓEVs were significantly associated to the patient's survival time.
Conclusions: An evolving cEV profile able to discriminate prompt risk of death during hospitalization has been defined suggesting a role for circulating and vascular cell-derived EVs in COVID-19 pathogenesis.
(© 2024 Stichting European Society for Clinical Investigation Journal Foundation. Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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