The diagnostic value of peripheral blood lymphocyte testing in children with infectious mononucleosis.

Autor: Zhou J; Department of Hematology, Suqian First Hospital, No. 120, Suzhi Road, Sucheng District, Suqian City, Jiangsu Province, 223800, China., Zhang J; Department of Pediatrics, Suqian First Hospital, No. 120, Suzhi Road, Sucheng District, Suqian City, Jiangsu Province, 223800, China., Zhu D; Department Of Clinical Laboratory, Suqian Children's Hospital, No. 1, Qinghai Lake Road, Sucheng District, Suqian City, Jiangsu Province, 223800, China., Ma W; Intensive Care Unit, Suqian First Hospital, No. 120, Suzhi Road, Sucheng District, Suqian City, Jiangsu Province, 223800, China., Zhong Q; Hematology Laboratory, Suqian First Hospital, No. 120, Suzhi Road, Sucheng District, Suqian City, Jiangsu Province, 223800, China., Shen Q; Department of Pediatrics, Suqian First Hospital, No. 120, Suzhi Road, Sucheng District, Suqian City, Jiangsu Province, 223800, China. shenqin2028@163.com., Su J; Hematology Laboratory, Suqian First Hospital, No. 120, Suzhi Road, Sucheng District, Suqian City, Jiangsu Province, 223800, China. 928007387@qq.com.; The Suqian Clinical College of Xuzhou Medical University, No. 209, Tongshan Road, Xuzhou City, Jiangsu Province, 221004, China. 928007387@qq.com.
Jazyk: angličtina
Zdroj: BMC pediatrics [BMC Pediatr] 2024 Nov 16; Vol. 24 (1), pp. 746. Date of Electronic Publication: 2024 Nov 16.
DOI: 10.1186/s12887-024-05228-6
Abstrakt: Objective: To investigate the diagnostic value of peripheral blood lymphocyte testing in children with infectious mononucleosis (IM).
Methods: A total of 135 children with IM as the IM group and 100 healthy volunteers as the healthy group were included in this retrospective study. Peripheral blood lymphocyte subsets marked as CD3+, CD4+, CD8+, CD16 + CD56+, and CD19 + in the peripheral blood were quantified using flow cytometry. Statistical analysis was performed using the chi-square test, Kruskal-Wallis test, AUROC curve, and Kappa consistency test to assess the diagnostic value of these markers in IM.
Results: The AUROC curve for CD8 + cells and for CD4+/CD8 + ratios both achieved a value of 1 with the sensitivity and specificity of 100% (P<0.001). The Kappa coefficients were 1 for CD8+, CD4+/CD8 + ratios and the combined EBV analysis, indicating a 100% consistency with the clinical diagnosis. Significant differences were also observed in the CD3+, CD4+, CD16 + CD56+, and CD19 + lymphocyte subsets between the IM group and the healthy group (P<0.05).
Conclusion: The evaluation of CD8 + and CD4+/CD8 + ratios in peripheral blood lymphocytes represents a significant advancement in the diagnosis of IM. Peripheral blood lymphocyte testing offers a reliable, sensitive, and specific diagnostic tool to enhance the clinical management of children with IM.
Competing Interests: Declarations Consent for publication Not Applicable. Human research ethics approval The study was approved by the Medical Ethics Committee of the Suqian First Hospital and Xuzhou Medical University (2019-SL-0014) and conducted in accordance with the Declaration of Helsinki. All patients’ parents or legal guardians were informed with the study and the signed consent forms were obtained. Consent to participate statement All patients’ parents or legal guardians were informed with the study and the signed consent forms were obtained. Competing interests The authors declare no competing interests.
(© 2024. The Author(s).)
Databáze: MEDLINE
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