Enabling biocontained plant virus transmission studies through establishment of an axenic whitefly (Bemisia tabaci) colony on plant tissue culture.

Autor: Thompson NS; Department of Chemical Engineering, The Pennsylvania State University, University Park, PA, 16802, USA., Krum D; Department of Chemical Engineering, The Pennsylvania State University, University Park, PA, 16802, USA., Chen YR; Department of Chemical Engineering, The Pennsylvania State University, University Park, PA, 16802, USA., Torres MC; Department of Chemical Engineering, The Pennsylvania State University, University Park, PA, 16802, USA.; Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA, 16802, USA., Trauger MA; Department of Chemical Engineering, The Pennsylvania State University, University Park, PA, 16802, USA., Strike D; Department of Chemical Engineering, The Pennsylvania State University, University Park, PA, 16802, USA., Weston Z; Department of Chemical Engineering, The Pennsylvania State University, University Park, PA, 16802, USA., Polston JE; Department of Plant Pathology, University of Florida, Gainesville, FL, 32611, USA., Curtis WR; Department of Chemical Engineering, The Pennsylvania State University, University Park, PA, 16802, USA. wrc2@psu.edu.; Intercollege Program in Plant Biology, The Pennsylvania State University, University Park, PA, 16802, USA. wrc2@psu.edu.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Nov 15; Vol. 14 (1), pp. 28169. Date of Electronic Publication: 2024 Nov 15.
DOI: 10.1038/s41598-024-73583-6
Abstrakt: Whiteflies (Bemisia tabaci) and the diseases they transmit are a major detriment to crop yields and a significant contributor to world hunger. The highly evolved interactions of host plant, phloem-feeding insect vector with endosymbionts and persistently transmitted virus represent a tremendous challenge for interdisciplinary study. Presented here is the establishment of a colony of axenic whiteflies on tissue-cultured plants. Efficient colony establishment was achieved by a surface sterilization of eggs laid on axenic phototrophically tissue-cultured plants. The transfer of emerging whiteflies through coupled tissue culture vessels to new axenic plants facilitates robust subculturing and produces hundreds of whitefly adults per month. Whitefly proliferation on more than two dozen plant species is shown as well as in vitro testing of whitefly preference for different plants. This novel multi-organism system provides the high-level of biocontainment required by Federal permitting to conduct virus transmission experiments. Axenic whitefly adults were able to acquire and transmit a begomovirus into tissue-cultured plants, indicating that culturable gut microorganisms are not required for virus transmission. The approach described enables a wide range of hypotheses regarding whitefly phytopathology without the expense, facilities, and contamination ambiguity associated with current approaches.
Competing Interests: Declarations Competing interests The authors declare no competing interests.
(© 2024. The Author(s).)
Databáze: MEDLINE
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