Antidiabetic and antioxidant profiling of 67 African trifoliate yam accessions by planar on-surface assays versus in vitro assays.

Autor: Aiyedun PO; Department of Pharmacognosy, Faculty of Pharmacy, University of Ibadan, Oduduwa Road, 200132 Ibadan, Oyo, Nigeria; Genetic Resources Centre, International Institute of Tropical Agriculture, Oyo Road, 200001 Ibadan, Oyo, Nigeria; Institute of Biology and Environmental Sciences, Carl von Ossietzky University Oldenburg, Carl-von-Ossietzky-Str. 9-11, 26129 Oldenburg, Germany., Sonibare MA; Department of Pharmacognosy, Faculty of Pharmacy, University of Ibadan, Oduduwa Road, 200132 Ibadan, Oyo, Nigeria., Gueye B; Genetic Resources Centre, International Institute of Tropical Agriculture, Oyo Road, 200001 Ibadan, Oyo, Nigeria., Albach DC; Institute of Biology and Environmental Sciences, Carl von Ossietzky University Oldenburg, Carl-von-Ossietzky-Str. 9-11, 26129 Oldenburg, Germany., Heil J; Institute of Nutritional Science, Chair of Food Science, Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany., Morlock GE; Institute of Nutritional Science, Chair of Food Science, Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany; Center for Sustainable Food Systems, Justus Liebig University Giessen, Senckenbergstr. 3, 35390 Giessen, Germany. Electronic address: Gertrud.Morlock@uni-giessen.de.
Jazyk: angličtina
Zdroj: Fitoterapia [Fitoterapia] 2024 Nov 14; Vol. 180, pp. 106299. Date of Electronic Publication: 2024 Nov 14.
DOI: 10.1016/j.fitote.2024.106299
Abstrakt: Trifoliate yam (Dioscorea dumetorum) is traditionally used to treat diabetics in Nigeria. However, almost no information is available on its antidiabetic constituents and their natural variance. Hence, the activity of methanolic tuber extracts of 67 trifoliate yam accessions from the largest collection in Africa was proven by four colorimetric antidiabetic and antioxidant in vitro assays, as diabetes is also linked with oxidative stress. For the first time, selected accessions were also analyzed by planar bioactivity profiling. It has a comparatively higher, more differentiated information content, is more sustainable in terms of material consumption, and enables straightforward compound prioritization and characterization. Up to a dozen individual antioxidant zones were revealed as well as one prominent zone inhibiting α-glucosidase and α-amylase. The latter inhibition zone was tentatively assigned to palmitic, linoleic, oleic, linolenic, oxo-nonanoic fatty acids by direct elution to heated electrospray ionization high-resolution mass spectrometry.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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