Archaeal type six secretion system mediates contact-dependent antagonism.

Autor:	Zachs T; Department of Biology, Institute of Molecular Biology & Biophysics, Eidgenössische Technische Hochschule Zürich, Otto-Stern-Weg 5, 8093 Zürich, Switzerland., Malit JJL; Department of Biology, Institute of Molecular Biology & Biophysics, Eidgenössische Technische Hochschule Zürich, Otto-Stern-Weg 5, 8093 Zürich, Switzerland., Xu J; Department of Biology, Institute of Molecular Biology & Biophysics, Eidgenössische Technische Hochschule Zürich, Otto-Stern-Weg 5, 8093 Zürich, Switzerland., Schürch A; Department of Biology, Institute of Molecular Biology & Biophysics, Eidgenössische Technische Hochschule Zürich, Otto-Stern-Weg 5, 8093 Zürich, Switzerland., Sivabalasarma S; Molecular Biology of Archaea, Institute of Biology, Faculty of Biology, University of Freiburg, Schänzlestr. 1, 79104 Freiburg, Germany.; Spemann Graduate School of Biology and Medicine, University of Freiburg, 79104 Freiburg, Germany., Nußbaum P; Molecular Biology of Archaea, Institute of Biology, Faculty of Biology, University of Freiburg, Schänzlestr. 1, 79104 Freiburg, Germany., Albers SV; Molecular Biology of Archaea, Institute of Biology, Faculty of Biology, University of Freiburg, Schänzlestr. 1, 79104 Freiburg, Germany., Pilhofer M; Department of Biology, Institute of Molecular Biology & Biophysics, Eidgenössische Technische Hochschule Zürich, Otto-Stern-Weg 5, 8093 Zürich, Switzerland.
Jazyk:	angličtina
Zdroj:	Science advances [Sci Adv] 2024 Nov 15; Vol. 10 (46), pp. eadp7088. Date of Electronic Publication: 2024 Nov 15.
DOI:	10.1126/sciadv.adp7088
Abstrakt:	Microbial communities are shaped by cell-cell interactions. Although archaea are often found in associations with other microorganisms, the mechanisms structuring these communities are poorly understood. Here, we report on the structure and function of haloarchaeal contractile injection systems (CISs). Using a combination of functional assays and time-lapse imaging, we show that Halogeometricum borinquense exhibits antagonism toward Haloferax volcanii by inducing cell lysis and inhibiting proliferation. This antagonism is contact-dependent and requires a functional CIS, which is encoded by a gene cluster that is associated with toxin-immunity pairs. Cryo-focused ion beam milling and imaging by cryo-electron tomography revealed that these CISs are bound to the cytoplasmic membrane, resembling the bacterial type six secretion systems (T6SSs). We show that related T6SS gene clusters are conserved and expressed in other haloarchaeal strains, which exhibit antagonistic behavior. Our data provide a mechanistic framework for understanding how archaea may shape microbial communities and affect the food webs they inhabit.
Databáze:	MEDLINE
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