MosAIC: An annotated collection of mosquito-associated bacteria with high-quality genome assemblies.

Autor: Foo A; Department of Vector Biology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom., Brettell LE; Department of Vector Biology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom.; School of Science, Engineering and Environment, University of Salford, Manchester, United Kingdom., Nichols HL; Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin, United States of America., Medina Muñoz M; Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin, United States of America., Lysne JA; Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin, United States of America., Dhokiya V; Department of Vector Biology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom., Hoque AF; Department of Vector Biology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom., Brackney DE; Department of Entomology, Connecticut Agricultural Experiment Station, New Haven, Connecticut, United States of America.; Center for Vector Biology and Zoonotic Diseases, Connecticut Agricultural Experiment Station, New Haven, Connecticut, United States of America., Caragata EP; Florida Medical Entomology Laboratory, Department of Entomology and Nematology, Institute of Food and Agricultural Sciences, University of Florida, Vero Beach, Florida, United States of America., Hutchinson ML; Division of Vector Management, Pennsylvania Department of Environmental Protection, Harrisburg, Pennsylvania, United States of America.; Division of Plant Health, Pennsylvania Department of Agriculture, Harrisburg, Pennsylvania, United States of America., Jacobs-Lorena M; Department of Molecular Microbiology and Immunology, Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, United States of America., Lampe DJ; Department of Biological Sciences, Duquesne University, Pittsburgh, Pennsylvania, United States of America., Martin E; Universite Claude Bernard Lyon 1, Laboratoire d'Ecologie Microbienne, UMR CNRS 5557, UMR INRAE 1418, VetAgro Sup, 69622 Villeurbanne, France., Valiente Moro C; Universite Claude Bernard Lyon 1, Laboratoire d'Ecologie Microbienne, UMR CNRS 5557, UMR INRAE 1418, VetAgro Sup, 69622 Villeurbanne, France., Povelones M; Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, United States of America., Short SM; Department of Entomology, The Ohio State University, Columbus, Ohio, United States of America., Steven B; Department of Environmental Science and Forestry, Connecticut Agricultural Experiment Station, New Haven, Connecticut, United States of America., Xu J; Department of Biology, New Mexico State University, Las Cruces, New Mexico, United States of America., Paustian TD; Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin, United States of America., Rondon MR; Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin, United States of America., Hughes GL; Department of Vector Biology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom.; Department of Tropical Disease Biology, Centre for Neglected Tropical Disease, Liverpool School of Tropical Medicine, Liverpool, United Kingdom., Coon KL; Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin, United States of America., Heinz E; Department of Vector Biology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom.; Department of Clinical Sciences, Liverpool School of Tropical Medicine, Liverpool, United Kingdom.; Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United Kingdom.
Jazyk: angličtina
Zdroj: PLoS biology [PLoS Biol] 2024 Nov 15; Vol. 22 (11), pp. e3002897. Date of Electronic Publication: 2024 Nov 15 (Print Publication: 2024).
DOI: 10.1371/journal.pbio.3002897
Abstrakt: Mosquitoes transmit medically important human pathogens, including viruses like dengue virus and parasites such as Plasmodium spp., the causative agent of malaria. Mosquito microbiomes are critically important for the ability of mosquitoes to transmit disease-causing agents. However, while large collections of bacterial isolates and genomic data exist for vertebrate microbiomes, the vast majority of work in mosquitoes to date is based on 16S rRNA gene amplicon data that provides limited taxonomic resolution and no functional information. To address this gap and facilitate future studies using experimental microbiome manipulations, we generated a bacterial Mosquito-Associated Isolate Collection (MosAIC) consisting of 392 bacterial isolates with extensive metadata and high-quality draft genome assemblies that are publicly available, both isolates and sequence data, for use by the scientific community. MosAIC encompasses 142 species spanning 29 bacterial families, with members of the Enterobacteriaceae comprising 40% of the collection. Phylogenomic analysis of 3 genera, Enterobacter, Serratia, and Elizabethkingia, reveal lineages of mosquito-associated bacteria isolated from different mosquito species in multiple laboratories. Investigation into species' pangenomes further reveals clusters of genes specific to these lineages, which are of interest for future work to test for functions connected to mosquito host association. Altogether, we describe the generation of a physical collection of mosquito-associated bacterial isolates, their genomic data, and analyses of selected groups in context of genome data from closely related isolates, providing a unique, highly valuable resource for research on bacterial colonisation and adaptation within mosquito hosts. Future efforts will expand the collection to include broader geographic and host species representation, especially from individuals collected from field populations, as well as other mosquito-associated microbes, including fungi, archaea, and protozoa.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Foo et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje