Generating iAstrocytes From Human Induced Pluripotent Stem Cells by Combining Low-Density Passaging of Neural Progenitor Cells and Transcription Factor NFIA Transdifferentiation.

Autor: Bosco P; Taub Institute for Research on Alzheimer's Disease and the Aging Brain, Columbia University Irving Medical Center, New York, New York., Akcan U; Department of Neurology, Columbia University Irving Medical Center, New York, New York., Williams D; Department of Neurology, Columbia University Irving Medical Center, New York, New York.; Center for Translational Research in Neurodevelopmental Disease, Columbia University Irving Medical Center, New York, New York., Buchanan HM; Department of Pathology and Cell Biology, Columbia University Irving Medical Center, New York, New York., Agalliu D; Department of Neurology, Columbia University Irving Medical Center, New York, New York.; Department of Pathology and Cell Biology, Columbia University Irving Medical Center, New York, New York., Sproul AA; Taub Institute for Research on Alzheimer's Disease and the Aging Brain, Columbia University Irving Medical Center, New York, New York.; Department of Pathology and Cell Biology, Columbia University Irving Medical Center, New York, New York.
Jazyk: angličtina
Zdroj: Current protocols [Curr Protoc] 2024 Nov; Vol. 4 (11), pp. e70049.
DOI: 10.1002/cpz1.70049
Abstrakt: Astrocytes are key regulators of central nervous system (CNS) homeostasis, and their dysfunction is implicated in neurological and neurodegenerative disorders. Here, we describe a two-step protocol to generate astrocytes from human induced pluripotent stem cells (hiPSCs) using a bankable neural progenitor cell (NPC) intermediate, followed by low-density passaging and overexpression of the gliogenic transcription factor NFIA. A bankable NPC intermediate allows for facile differentiation into both purified neuronal and astrocyte cell types in parallel from the same genetic background, depending on the experimental needs. This article presents a protocol to generate NPCs from hiPSCs, which are then differentiated into hiPSC-derived astrocytes, termed iAstrocytes. The resulting iAstrocytes express key markers of astrocyte identity at transcript and protein levels by bulk RNA-Seq and immunocytochemistry, respectively. Additionally, they respond to the inflammatory stimuli poly(I:C) and generate waves of calcium activity in response to either physical activity or the addition of ATP. Our approach offers a simple and robust method to generate and characterize human astrocytes, which can be used to model human disease affecting this cell type. © 2024 Wiley Periodicals LLC. Basic Protocol 1: Differentiation of hiPSCs to NPCs Basic Protocol 2: Differentiation of NPCs into iAstrocytes Support Protocol 1: Molecular validation of iAstrocytes Support Protocol 2: Calcium imaging-based validation of iAstrocyte function Support Protocol 3: Differentiation of NPCs into neurons.
(© 2024 Wiley Periodicals LLC.)
Databáze: MEDLINE
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