Metabolic Phenotyping of Synaptic Mitochondria Using MitoPlates™ and Synaptoneurosomes.

Autor: Stawikowska A; Department of Animal Physiology, Faculty of Biology, University of Warsaw, Warsaw, Poland., Dziembowska M; Department of Animal Physiology, Faculty of Biology, University of Warsaw, Warsaw, Poland., Kuzniewska B; Department of Animal Physiology, Faculty of Biology, University of Warsaw, Warsaw, Poland. bozena.kuzniewska@uw.edu.pl.
Jazyk: angličtina
Zdroj: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2025; Vol. 2878, pp. 67-74.
DOI: 10.1007/978-1-0716-4264-1_4
Abstrakt: Mitochondrial functional assays using MitoPlates™ S-1 allow us to characterize mitochondria in terms of substrate metabolism. MitoPlates™ are 96-well microplates pre-coated with a diverse set of substrates. The electron flow from NADH and FADH 2 producing mitochondrial substrates is measured based on the reduction of redox dye, that acts as a terminal electron acceptor. Here, we describe the application of MitoPlates™ to characterize the metabolism of synaptic mitochondria enclosed in isolated pre- and postsynaptic terminals (synaptoneurosomes).
(© 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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