Mouse Testing Methods in Psychoneuroimmunology: Measuring Behavioral Responses.

Autor:	Krauklis SA; Department of Animal Sciences, University of Illinois at Urbana-Champaign, Champaign, IL, USA.; Division of Nutritional Sciences, University of Illinois at Urbana-Champaign, Champaign, IL, USA., Towers AE; Division of Nutritional Sciences, University of Illinois at Urbana-Champaign, Champaign, IL, USA., York JM; Department of Anatomy and Cell Biology, University of Illinois at Chicago, Chicago, IL, USA., Baynard T; Academic Affairs, University of Massachusetts-Boston, Boston, MA, USA., Gainey SJ; Department of Animal Sciences, University of Illinois at Urbana-Champaign, Champaign, IL, USA., Freund GG; Department of Pathology, Roswell Park Comprehensive Cancer Center, Buffalo, NY, USA., Steelman AJ; Department of Animal Sciences, University of Illinois at Urbana-Champaign, Champaign, IL, USA. asteelma@illinois.edu.; Division of Nutritional Sciences, University of Illinois at Urbana-Champaign, Champaign, IL, USA. asteelma@illinois.edu.; Neuroscience Program, University of Illinois at Urbana-Champaign, Champaign, IL, USA. asteelma@illinois.edu.; Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Champaign, IL, USA. asteelma@illinois.edu.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2025; Vol. 2868, pp. 163-203.
DOI:	10.1007/978-1-0716-4200-9_10
Abstrakt:	The field of psychoneuroimmunology (PNI) aims to uncover the processes and consequences of nervous, immune, and endocrine system relationships. Behavior is a consequence of such interactions and manifests from a complex interweave of factors including immune-to-neural and neural-to-immune communication. Often the signaling molecules involved during a particular episode of neuroimmune activation are not known, but behavioral response provides evidence that bioactives such as neurotransmitters and cytokines are perturbed. Immunobehavioral phenotyping is a first-line approach when examining the neuroimmune system and its reaction to immune stimulation or suppression. Behavioral response is significantly more sensitive than direct measurement of a single specific bioactive and can quickly and efficiently rule in or out relevance of a particular immune challenge or therapeutic to neuroimmunity. Classically, immunobehavioral research was focused on sickness symptoms related to bacterial infection, but neuroimmune activation is now a recognized complication of diseases and disorders ranging from cancer to diabesity to Alzheimer's. Immunobehaviors include lethargy, loss of appetite, and disinterest in social activity/surrounding environment. In addition, neuroimmune activation can diminish physical activity, precipitate feelings of depression and anxiety, and impair cognitive and executive function. Provided is a detailed overview of behavioral tests frequently used to examine neuroimmune activation in mice with a special emphasis on pre-experimental conditions that can confound or prevent successful immunobehavioral experimentation. (© 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu