Exosome-mediated viral nucleic acid presentation in a crustacean expounds innate immunity from a novel perspective.

Autor:	Gong Y; Guangdong Provincial Key Laboratory of Marine Biology, Shantou University, Shantou, China.; School of Life Sciences, Nanchang University, Nanchang, China., Hu H; Guangdong Provincial Key Laboratory of Marine Biology, Shantou University, Shantou, China., Zhao X; Guangdong Provincial Key Laboratory of Marine Biology, Shantou University, Shantou, China., Wei W; Guangdong Provincial Key Laboratory of Marine Biology, Shantou University, Shantou, China., Zhang M; Guangdong Provincial Key Laboratory of Marine Biology, Shantou University, Shantou, China.; Institute of Marine Sciences, Shantou University, Shantou, China., Tran NT; Guangdong Provincial Key Laboratory of Marine Biology, Shantou University, Shantou, China.; Institute of Marine Sciences, Shantou University, Shantou, China., Ma H; Guangdong Provincial Key Laboratory of Marine Biology, Shantou University, Shantou, China.; Institute of Marine Sciences, Shantou University, Shantou, China., Zhang Y; Guangdong Provincial Key Laboratory of Marine Biology, Shantou University, Shantou, China.; Institute of Marine Sciences, Shantou University, Shantou, China., Chan K-G; Guangdong Provincial Key Laboratory of Marine Biology, Shantou University, Shantou, China.; Division of Genetics and Molecular Biology, Institute of Biological Science, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia., Li S; Guangdong Provincial Key Laboratory of Marine Biology, Shantou University, Shantou, China.; Institute of Marine Sciences, Shantou University, Shantou, China.
Jazyk:	angličtina
Zdroj:	Journal of virology [J Virol] 2024 Dec 17; Vol. 98 (12), pp. e0151924. Date of Electronic Publication: 2024 Nov 15.
DOI:	10.1128/jvi.01519-24
Abstrakt:	As an enduring hot topic in the field of innate immunity, apoptosis is widely considered an effective approach to eliminate pathogenic microbes and plays a crucial role during host-pathogen interactions. Recently, researchers have found that the virus-containing host cells could transmit apoptotic signals to the surrounding uninfected cells during infection, but the mechanism remains unclear. Here, we found that exosomes secreted by WSSV-infected mud crab hemocytes contain viral nucleic acid wsv277, which could be transported to the recipient cells and further expressed viral protein with phosphokinase activity. Besides, by using transcriptome, proteome, ChIP-seq, and coIP techniques, the results revealed that wsv277 could activate the transcription and translation of apoptotic genes via interacting with CBF and EF-1α so as to suppress the spread of virus infection by inducing apoptosis of the surrounding cells. Therefore, for the first time, our study proved that the components of DNA virus could be encapsulated into exosomes and elucidated the mechanism of apoptotic signal transduction between cells from the perspective of exosomes. Importance: Our study revealed that the components of DNA virus could be packaged and transmitted through the exosomes of lower invertebrates, which strongly demonstrated the diversity of exosome-mediated viral immunity and its universality in animals. Furthermore, we elucidated the mechanism of apoptotic signal transduction between cells from the perspective of exosomes and revealed a novel strategy for the host to cope with viral infection. Competing Interests: The authors declare no conflict of interest.
Databáze:	MEDLINE
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