Morinda officinalis saponins promote osteogenic differentiation of human umbilical cord-derived mesenchymal stem cells via the BMP-SMAD signaling pathway.
| Autor: | Zhou J; Applied Biology Laboratory, College of Pharmaceutical and Biological Engineering, Shenyang University of Chemical Technology Shenyang 110142, Liaoning, China.; College of Pharmaceutical Sciences, Gannan Medical University Ganzhou 341000, Jiangxi, China., Zhou F; Applied Biology Laboratory, College of Pharmaceutical and Biological Engineering, Shenyang University of Chemical Technology Shenyang 110142, Liaoning, China., Yang L; Applied Biology Laboratory, College of Pharmaceutical and Biological Engineering, Shenyang University of Chemical Technology Shenyang 110142, Liaoning, China., Liang H; Applied Biology Laboratory, College of Pharmaceutical and Biological Engineering, Shenyang University of Chemical Technology Shenyang 110142, Liaoning, China., Zhu Q; Applied Biology Laboratory, College of Pharmaceutical and Biological Engineering, Shenyang University of Chemical Technology Shenyang 110142, Liaoning, China., Guo F; Glabiolus Biotech (Xuzhou) Co., Ltd. Xuzhou 221000, Jiangsu, China., Yin X; Applied Biology Laboratory, College of Pharmaceutical and Biological Engineering, Shenyang University of Chemical Technology Shenyang 110142, Liaoning, China., Li J; College of Pharmaceutical Sciences, Gannan Medical University Ganzhou 341000, Jiangxi, China.; Glabiolus Biotech (Jiangxi) Co., Ltd. Ganzhou 341005, Jiangxi, China. |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | American journal of translational research [Am J Transl Res] 2024 Oct 15; Vol. 16 (10), pp. 5441-5453. Date of Electronic Publication: 2024 Oct 15 (Print Publication: 2024). |
| DOI: | 10.62347/KNRS3234 |
| Abstrakt: | Background: Morinda officinalis saponins (MOS), a traditional Chinese medicine extracted from M. officinalis roots, have been used as a health supplement. Existing evidence suggests that extracts from this plant can be used for osteoporosis treatment. However, the molecular mechanisms underlying the anti-osteoporotic effects of M. officinalis remain poorly understood. Methods and Results: In this study, we investigated the osteogenesis-promoting effects of MOS on human umbilical cord-derived mesenchymal stem cells (HUC-MSCs). Alkaline phosphatase staining, alizarin red staining, and quantitative reverse transcription-PCR demonstrated that MOS promoted the osteogenic differentiation of HUC-MSCs in a concentration-dependent manner. RNA sequencing results showed that the expression of key osteogenic differentiation-related genes, including BMP4 , as well as the activity of transforming growth factor-β and calcium signaling pathways increased following MOS treatment. Furthermore, treatment with the bone morphogenetic protein (BMP) antagonist Noggin reversed the MOS-induced pro-osteogenic differentiation effects and the upregulation of osteoblast-specific markers. Conclusions: Overall, the results indicate that MOS can partially promote osteogenic differentiation of HUC-MSCs by regulating the BMP-SMAD signaling pathway. These findings indicate the potential utility of MOS as a therapeutic agent for osteoporosis, particularly in the context of stem cell therapy. Competing Interests: None. (AJTR Copyright © 2024.) |
| Databáze: | MEDLINE |
| Externí odkaz: |
|