Analysis of the genome-editing activity of microinjected CRISPR/Cas9 ribonucleoprotein complexes in Daphnia pulex .
| Autor: | Maar ME; Biology, Indiana University, Bloomington, Indiana, United States., Miller JC; Biology, Indiana University, Bloomington, Indiana, United States., Lynch M; Center for Mechanisms of Evolution, Arizona State University, Tempe, Arizona, United States., Zelhof AC; Biology, Indiana University, Bloomington, Indiana, United States. |
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| Jazyk: | angličtina |
| Zdroj: | MicroPublication biology [MicroPubl Biol] 2024 Oct 30; Vol. 2024. Date of Electronic Publication: 2024 Oct 30 (Print Publication: 2024). |
| DOI: | 10.17912/micropub.biology.001310 |
| Abstrakt: | Although Daphnia is a widely used model organism with a completely sequenced genome, molecular tools for analyzing specific gene functions are still being developed. Progress has been made in developing CRISPR/Cas9 gene editing in Daphnia . However, the gene-editing activity of injected ribonucleoprotein complexes (RNPs), the success of co-injected RNPs with different gRNAs, and the heritability of mutations in asexual progeny need further investigation. Here, we show prolonged Cas9 RNP activity past the one-cell stage injected individuals, leading to a wide range of somatic mutations, and germline mosaicism of heritable biallelic mutations. Competing Interests: The authors declare that there are no conflicts of interest present. (Copyright: © 2024 by the authors.) |
| Databáze: | MEDLINE |
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