Activation of the Innate Immune System in Brain-Dead Donors Can Be Reduced by Luminal Intestinal Preservation During Organ Procurement Surgery - A Porcine Model.

Autor:	Weiss MG; Department of Nephrology, Aarhus University Hospital, Aarhus, Denmark.; Department of Clinical Medicine, Aarhus University, Aarhus, Denmark., de Jong AM; Department of Gastroenterology and Hepatology, University Medical Centre Groningen, University of Groningen, Groningen, Netherlands., Seegert H; Department of Clinical Medicine, Aarhus University, Aarhus, Denmark., Moeslund N; Department of Clinical Medicine, Aarhus University, Aarhus, Denmark.; Department of Cardiology, Aarhus University Hospital, Aarhus, Denmark., Maassen H; Department of Surgery, University Medical Centre Groningen, University of Groningen, Groningen, Netherlands., Schjalm C; Department of Immunology, Oslo University Hospital, Oslo, Norway.; Institute of Clinical Medicine, University of Oslo, Oslo, Norway., de Boer E; Department of Immunology, Oslo University Hospital, Oslo, Norway.; Institute of Clinical Medicine, University of Oslo, Oslo, Norway., Leuvenink H; Department of Surgery, University Medical Centre Groningen, University of Groningen, Groningen, Netherlands., Mollnes TE; Department of Immunology, Oslo University Hospital, Oslo, Norway.; Institute of Clinical Medicine, University of Oslo, Oslo, Norway.; Research Laboratory, Nordland Hospital, Bodø, Norway., Eijken M; Department of Nephrology, Aarhus University Hospital, Aarhus, Denmark.; Department of Clinical Medicine, Aarhus University, Aarhus, Denmark.; Department of Immunology, Aarhus University Hospital, Aarhus, Denmark., Keller AK; Department of Clinical Medicine, Aarhus University, Aarhus, Denmark.; Department of Urology, Aarhus University Hospital, Aarhus, Denmark., Dijkstra G; Department of Gastroenterology and Hepatology, University Medical Centre Groningen, University of Groningen, Groningen, Netherlands., Jespersen B; Department of Nephrology, Aarhus University Hospital, Aarhus, Denmark.; Department of Clinical Medicine, Aarhus University, Aarhus, Denmark., Pischke SE; Department of Immunology, Oslo University Hospital, Oslo, Norway.; Institute of Clinical Medicine, University of Oslo, Oslo, Norway.; Department of Anaesthesiology and Intensive Care, Oslo University Hospital, Oslo, Norway.
Jazyk:	angličtina
Zdroj:	Transplant international : official journal of the European Society for Organ Transplantation [Transpl Int] 2024 Oct 31; Vol. 37, pp. 13569. Date of Electronic Publication: 2024 Oct 31 (Print Publication: 2024).
DOI:	10.3389/ti.2024.13569
Abstrakt:	Organs obtained from brain dead donors can have suboptimal outcomes. Activation of the innate immune system and translocation of intestinal bacteria could be causative. Thirty two pigs were assigned to control, brain death (BD), BD + luminal intestinal polyethylene glycol (PEG), and BD + luminal intestinal University of Wisconsin solution (UW) groups. Animals were observed for 360 min after BD before organ retrieval. 2,000 mL luminal intestinal preservation solution was instilled into the duodenum at the start of organ procurement. Repeated measurements of plasma C3a, Terminal Complement Complex (TCC), IL-8, TNF, and lipopolysaccharide binding protein were analysed by immunoassays. C3a was significantly higher in the BD groups compared to controls at 480 min after brain death. TCC was significantly higher in BD and BD + UW, but not BD + PEG, compared to controls at 480 min. TNF was significantly higher in the BD group compared to all other groups at 480 min. LPS binding protein increased following BD in all groups except BD + PEG, which at 480 min was significantly lower compared with all other groups. Brain death induced innate immune system activation was decreased by luminal preservation using PEG during organ procurement, possibly due to reduced bacterial translocation. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Weiss, de Jong, Seegert, Moeslund, Maassen, Schjalm, de Boer, Leuvenink, Mollnes, Eijken, Keller, Dijkstra, Jespersen and Pischke.)
Databáze:	MEDLINE
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